免疫磁珠分离技术在大肠杆菌O157∶H7检测中的应用

[Application of immunomagnetic separation to E. coli O157 : H7 detection].

作者信息

Tang Qian-Qian, Wang Jian-Ping, Ye Zun-Zhong, Gai Ling, Ying Yi-Bin

机构信息

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Oct;29(10):2614-8.

PMID:20038020

Abstract

In the present paper, the capture efficiencies of four different proportions of E. coli O157 : H7 and immunomagnetic beads (IMB) (1 : 6.34, 1 : 12.69, 1 : 25.38 and 1 : 30.07) and three different proportions of E. coli O157 : H7 and Bacillus subtilis (0.3 mL : 0.1 mL, 0.2 mL : 0.2 mL and 0.1 mL : 0.3 mL) were compared, with the total volume and capacity of IMB remaining constant. It was revealed that the capture efficiency could reach 100% when the proportion of E. coli O157 : H7 and IMB was 1 : 30, meaning that almost all targets E. coli O157 : H7 could be captured at 1 : 30, and that the capture efficiency decreased and then increased with the number of Bacillus subtilis increasing. Moreover, the combination of ATP bioluminescence and IMS was correlated well with conventional culture methods, R2 = 0.9882, and the detection limit was as low as 10(2) CFU x mL(-1).

摘要

在本论文中，比较了大肠杆菌O157 : H7与免疫磁珠（IMB）四种不同比例（1 : 6.34、1 : 12.69、1 : 25.38和1 : 30.07）以及大肠杆菌O157 : H7与枯草芽孢杆菌三种不同比例（0.3 mL : 0.1 mL、0.2 mL : 0.2 mL和0.1 mL : 0.3 mL）的捕获效率，IMB的总体积和容量保持不变。结果表明，当大肠杆菌O157 : H7与IMB的比例为1 : 30时，捕获效率可达100%，这意味着在1 : 30的比例下几乎所有目标大肠杆菌O157 : H7都能被捕获，并且捕获效率随着枯草芽孢杆菌数量的增加先降低后升高。此外，ATP生物发光与免疫磁分离技术（IMS）的结合与传统培养方法相关性良好，R2 = 0.9882，检测限低至10(2) CFU x mL(-1)。