Zhang Yun-Liang, Liu Yan-Cheng, Shi Shao-Ming, Chen Zhen-Feng, Liang Hong
The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, Guilin 541004, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Oct;29(10):2848-50.
Dry and wet methods were used to digest Phellodendron grown in Jinxiu Dayaoshan of Guangxi with a high-pressure jar and to prepare the samples. The contents of microelements Cu, Fe, Zn, Mn and Ni and macroelements K, Ca, Mg, Sr and Na in Phellodendron were determined by using ICP-AES. Meanwhile, the optimal digestion conditions were found: 7 mL of HNO3 +HClO4 +H2O2 (volume ratio 5 : 1 : 1) for 4 h. The work condition and the optical wavelength were selected. The RSD was found to be less than 5% and the recovery rates of all elements were within the range of 89.4%-113.1%. The determined results agree with the analytical demand, and also indicate that Phellodendron contains high contents of Cu, Fe, Zn and Mn microelements. It is important to assess the medicinal value and understand pharmacological action of Phellodendron by the determination of microelements in the Chinese medicine with ICP-AES.
采用干法和湿法，使用高压罐对广西金秀大瑶山种植的黄柏进行消解并制备样品。采用电感耦合等离子体发射光谱法（ICP-AES）测定黄柏中微量元素铜（Cu）、铁（Fe）、锌（Zn）、锰（Mn）、镍（Ni）以及常量元素钾（K）、钙（Ca）、镁（Mg）、锶（Sr）、钠（Na）的含量。同时，找出了最佳消解条件：7 mL硝酸 + 高氯酸 + 过氧化氢（体积比5 : 1 : 1），消解4 h。选择了工作条件和光学波长。相对标准偏差（RSD）小于5%，各元素回收率在89.4% - 113.1%范围内。测定结果符合分析要求，也表明黄柏中铜、铁、锌、锰等微量元素含量较高。通过电感耦合等离子体发射光谱法测定中药黄柏中的微量元素，对于评估其药用价值和了解药理作用具有重要意义。
