National Reference Laboratory of Veterinary Drug Residues/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
J Agric Food Chem. 2010 Jan 27;58(2):937-42. doi: 10.1021/jf902418k.
A residue depletion study was performed to investigate the tissue kinetics and tissue-plasma correlation of methyl-3-quinoxaline-2-carboxylic acid (MQCA), the marker residue of olaquindox (OLA), in pigs. Twenty-five pigs were randomly divided into a test and a control group. The former group was treated with 100 mg/kg OLA in its feed for 30 consecutive days, and the latter was given blank feed for the same period. One control and four treated animals were slaughtered at 0.5, 3, 10, 17, and 28 days post-medication. Muscle, liver, kidney, fat, and plasma samples were collected and analyzed using the validated high-performance liquid chromatography method (HPLC). Results showed that the tissue concentration of MQCA in the liver > kidney > fat > muscle, at almost all time points. The half-lives of MQCA in the muscle, liver, kidney, fat, and plasma were 12, 8, 15, 8, and 6 days, respectively. A withdrawal period of 38 days was calculated using the statistical method recommended by the European Medical Evaluation Agency (EMEA). Good correlations between tissue and plasma MQCA levels were found in the present study with correlation coefficients of more than 0.92. These correlations would be helpful in the routine monitoring of OLA in porcine tissues, without sacrificing the animals.
本研究进行了残留消除试验，旨在研究喹乙醇（OLA）标志物 3-甲基-2-喹喔啉羧酸（MQCA）在猪体内的组织动力学和组织-血浆相关性。将 25 头猪随机分为试验组和对照组。试验组连续 30 天经饲料摄入 100mg/kg 的 OLA，对照组同期给予空白饲料。在给药后 0.5、3、10、17 和 28 天，每组分别屠宰一头对照猪和四头处理猪。采集肌肉、肝脏、肾脏、脂肪和血浆样品，采用经验证的高效液相色谱法（HPLC）进行分析。结果表明，MQCA 在肝脏中的浓度>肾脏>脂肪>肌肉，几乎所有时间点均如此。MQCA 在肌肉、肝脏、肾脏、脂肪和血浆中的半衰期分别为 12、8、15、8 和 6 天。根据欧洲药品管理局（EMEA）推荐的统计方法，计算出 38 天的停药期。本研究中，MQCA 在组织和血浆中的水平之间存在良好的相关性，相关系数均大于 0.92。这些相关性有助于在不牺牲动物的情况下，对猪组织中的 OLA 进行常规监测。
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