Department of Oral and Maxillofacial Surgery, Sun Yat-Sen University, Guangzhou, China.
J Oral Pathol Med. 2010 Mar;39(3):263-8. doi: 10.1111/j.1600-0714.2009.00842.x. Epub 2009 Dec 16.
The aim of this study was to evaluate the roles of Notch signaling in the oncogenesis and cytodifferentiation of cemento-ossifying fibroma, the expressions of Notch receptors and ligands were detected in COF and normal jaw bones.
The expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry respectively in 16 cases of normal bone tissues and 12 cases of COF of the jaws.
The mRNAs expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected in all specimens. The expression levels of mRNAs in COF were higher than those in normal bones. In COF, Notch proteins staining were showed extensively distribution in fibroblasts and osteoblasts. In normal bone tissue, Notch proteins were expressed in osteoblasts, whereas proteins staining were weaker than those in COF, but no detection in fibroblast-like bone marrow stroma cells. The expressions of Notch receptors and ligands were not detected in cementum-like products or bone matrices.
Our data suggest that Notch signaling may participate in controlling cell differentiation and proliferation in normal bone and COF of the jaws. Notch signaling disorder may be a molecular incident in COF occurrence and development.
本研究旨在评估 Notch 信号通路在骨化性纤维瘤发生和细胞分化中的作用，检测 Notch 受体和配体在骨化性纤维瘤和正常颌骨中的表达。
采用逆转录聚合酶链反应和免疫组织化学方法检测 16 例正常颌骨组织和 12 例骨化性纤维瘤中 Notch1、Notch3、Jagged1 和 Jagged2 的表达。
所有标本均检测到 Notch1、Notch3、Jagged1 和 Jagged2 的 mRNA 表达。骨化性纤维瘤中的 mRNA 表达水平高于正常骨。在骨化性纤维瘤中，Notch 蛋白染色广泛分布于成纤维细胞和成骨细胞中。在正常骨组织中，Notch 蛋白在成骨细胞中表达，但染色强度弱于骨化性纤维瘤，而在成纤维样骨髓基质细胞中未检测到。在牙骨质样产物或骨基质中未检测到 Notch 受体和配体的表达。
我们的数据表明，Notch 信号通路可能参与调节正常骨和颌骨骨化性纤维瘤中细胞的分化和增殖。Notch 信号通路异常可能是骨化性纤维瘤发生发展的分子事件。
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