Sandia National Laboratories, Albuquerque, New Mexico 87123, USA.
Biomacromolecules. 2010 Feb 8;11(2):357-68. doi: 10.1021/bm9008952.
Small angle neutron scattering (SANS) was used to study the structure of Avicel (FD100) microcrystalline cellulose during enzymatic digestion. Digestions were performed in either of two modes: a static, quiescent mode or a dynamic mode using a stirred suspension recycled through a flow cell. The scattering pattern for as-received Avicel in D(2)O buffer is comprised of a low Q power law region resulting from the surface fractal character of the microcrystalline fibers and a high Q roll-off due to scattering from water-filled nanopores with radii approximately 20 A. For digestions in the dynamic mode the high Q roll-off decreased in magnitude within approximately 1 h after addition of enzymes, whereas in the static digestions no change was observed in the high Q roll-off, even after 60 h. These results indicate that only with significant agitation does enzyme digestion affect the structure of the nanopores.
小角中子散射（SANS）被用于研究微晶纤维素 Avicel（FD100）在酶解过程中的结构。酶解在两种模式下进行：一种是静态、静止模式，另一种是使用通过流动池循环的搅拌悬浮液的动态模式。在 D（2）O 缓冲液中，原始 Avicel 的散射图谱由低 Q 幂律区域组成，这是由于微晶纤维的表面分形特征所致，而由于半径约为 20 A 的充满水的纳米孔的散射，高 Q 衰减。在动态模式下，酶添加后约 1 小时内，高 Q 衰减的幅度减小，而在静态消化中，即使经过 60 小时，高 Q 衰减也没有变化。这些结果表明，只有在剧烈搅拌下，酶解才会影响纳米孔的结构。
