Djimde Abdoulaye A, Barger Breanna, Kone Aminatou, Beavogui Abdoul H, Tekete Mamadou, Fofana Bakary, Dara Antoine, Maiga Hamma, Dembele Demba, Toure Sekou, Dama Souleymane, Ouologuem Dinkorma, Sangare Cheick Papa Oumar, Dolo Amagana, Sogoba Nofomo, Nimaga Karamoko, Kone Yacouba, Doumbo Ogobara K
Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, Faculty of Medicine, Pharmacy and Odonto-Stomatology, University of Bamako, Mali, West Africa.
FEMS Immunol Med Microbiol. 2010 Feb;58(1):113-8. doi: 10.1111/j.1574-695X.2009.00641.x. Epub 2009 Nov 2.
Plasmodium falciparum chloroquine resistance (CQR) transporter point mutation (PfCRT 76T) is known to be the key determinant of CQR. Molecular detection of PfCRT 76T in field samples may be used for the surveillance of CQR in malaria-endemic countries. The genotype-resistance index (GRI), which is obtained as the ratio of the prevalence of PfCRT 76T to the incidence of CQR in a clinical trial, was proposed as a simple and practical molecular-based addition to the tools currently available for monitoring CQR in the field. In order to validate the GRI model across populations, time, and resistance patterns, we compiled data from the literature and generated new data from 12 sites across Mali. We found a mean PfCRT 76T mutation prevalence of 84.5% (range 60.9-95.1%) across all sites. CQR rates predicted from the GRI model were extrapolated onto a map of Mali to show the patterns of resistance throughout the participating regions. We present a comprehensive map of CQR in Mali, which strongly supports recent changes in drug policy away from chloroquine.
恶性疟原虫氯喹抗性（CQR）转运蛋白点突变（PfCRT 76T）已知是CQR的关键决定因素。对现场样本中PfCRT 76T进行分子检测可用于疟疾流行国家的CQR监测。基因型抗性指数（GRI）是在一项临床试验中通过PfCRT 76T的流行率与CQR发病率之比获得的，它被提议作为一种简单实用的基于分子的补充工具，用于目前在现场监测CQR的现有工具。为了在不同人群、时间和抗性模式中验证GRI模型，我们收集了文献数据并从马里的12个地点生成了新数据。我们发现所有地点的PfCRT 76T突变平均流行率为84.5%（范围60.9 - 95.1%）。从GRI模型预测的CQR率被外推到马里地图上，以显示整个参与地区的抗性模式。我们展示了一张马里CQR的综合地图，它有力地支持了最近远离氯喹的药物政策变化。
