Li Ying-Hui, Yuan Cui-Ping, Zhang Chen, Li Wei, Nan Hai-Yang, Chang Ru-Zhen, Qiu Li-Juan
National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Germplasm & Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Yi Chuan. 2009 Dec;31(12):1259-64. doi: 10.3724/sp.j.1005.2009.01259.
For clarifying the difference of genetic diversity and linkage disequilibrium (LD) level between cultivated (Glycine max (L.) Merr.) and annual wild soybean (Glycine soja Sieb. & Zucc.), genetic variation pattern of 8 SNP loci developed from soybean cyst nematode resistance candidate genes rhg1 and Rhg4 in soybean germplasm were analyzed. The results indicated that G. max population, consisted of cultivated soybean mini-core collection and modern cultivars, had a higher LD levels (R2 value is 0.216) than G. soja population. Since 100% of pairwise loci within a gene and 16.6% of pairwise loci between genes were significant in G. max population, two specific LD regions were formed for each gene. A total of 46 haplotypes were detected in 363 soybean germplasm. The population of G. soja had less number of haplotypes and higher haplotype diversity than the population of G. max. Among the 31 population-specific haplotypes, 15 haplotypes were specific for G. soja population. In addition, the frequency of two major predominant haplotypes (Hap_10 and Hap_11) in G. soja population was obviously decreased in G. max population, which might indicate that some new haplotypes were formed and some old haplotypes were lost during the G. max domesticated from G. soja.
为了阐明栽培大豆（Glycine max (L.) Merr.）和一年生野生大豆（Glycine soja Sieb. & Zucc.）之间遗传多样性和连锁不平衡（LD）水平的差异，分析了大豆种质中从大豆胞囊线虫抗性候选基因rhg1和Rhg4开发的8个SNP位点的遗传变异模式。结果表明，由栽培大豆微型核心种质库和现代品种组成的G. max群体的LD水平（R2值为0.216）高于G. soja群体。由于G. max群体中一个基因内的成对位点100%显著，基因间的成对位点16.6%显著，因此每个基因形成了两个特定的LD区域。在363份大豆种质中共检测到46种单倍型。G. soja群体的单倍型数量比G. max群体少，但单倍型多样性更高。在31种群体特异性单倍型中，有15种单倍型是G. soja群体特有的。此外，G. soja群体中两种主要优势单倍型（Hap_10和Hap_11）在G. max群体中的频率明显降低，这可能表明在G. soja驯化形成G. max的过程中形成了一些新的单倍型，同时一些旧的单倍型丢失了。
