Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul 120-752, Korea.
Int J Oncol. 2010 Feb;36(2):405-14.
We have identified an 8-gene signature with significant expression differences between gastric cancer and normal gastric tissues. This 8-gene set can predict the normal and cancer status of gastric tissues with more than 96% accuracy in a totally independent microarray dataset. The 8 genes are composed of down-regulated KLF4, GPX3, SST and LIPF, together with up-regulated SERPINH1, THY1 and INHBA in gastric cancer. To corroborate the differential gene expression pattern, we chose GPX3 and examined its expression pattern in detail. A comparison of GPX3 expression pattern shows a broader down-regulated pattern in multiple types of cancers, including cervical, thyroid, head and neck, lung cancers and melanoma than in healthy controls. An immuno-histostaining analysis in tissue microarrays confirms GPX3 down-regulation in gastric cancer. Mechanism-wise GPX3 down-regulation in gastric cancer is due to promoter hypermethylation. Collectively, these results show a correct identification of 8 genes as gastric cancer biomarkers.
我们已经确定了一个在胃癌和正常胃组织之间具有显著表达差异的 8 基因特征。在一个完全独立的微阵列数据集上，这个 8 基因集可以预测胃组织的正常和癌症状态，准确率超过 96%。这 8 个基因包括在胃癌中下调的 KLF4、GPX3、SST 和 LIPF，以及上调的 SERPINH1、THY1 和 INHBA。为了证实差异基因表达模式，我们选择了 GPX3 并详细检查了其表达模式。与健康对照组相比，GPX3 在多种癌症（包括宫颈癌、甲状腺癌、头颈部癌、肺癌和黑色素瘤）中的表达模式显示出更广泛的下调。组织微阵列的免疫组织化学分析证实了胃癌中 GPX3 的下调。从机制上讲，胃癌中 GPX3 的下调是由于启动子超甲基化。总的来说，这些结果表明正确识别了 8 个基因作为胃癌的生物标志物。
