Erskine Ronald J, Brockett Anne R, Beeching Nicole D, Hull Robert W, Bartlett Paul C
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Am J Vet Res. 2010 Jan;71(1):120-4. doi: 10.2460/ajvr.71.1.120.
To determine effects of injection site on antibody response to J5 Escherichia coli bacterin.
28 adult Holstein cows.
Cows were randomly assigned as control cattle (n = 4 cows), not administered J5 E coli bacterin; 3X (8), administered 3 doses of bacterin SC in the left side of the neck; 5XN (8), administered 5 doses of bacterin SC in the left side of the neck; or 5XSR (8), administered 5 doses of bacterin SC sequentially in the left side of the neck, right side of the neck, right side of the thorax, left side of the thorax, and left side of the neck. Blood samples were collected from the cows to determine anti-J5 E coli IgG1 and IgG2 concentrations.
Vaccinated cows had higher mean serum anti-J5 E coli IgG1 concentrations than did control cows. The 5XN and 5XSR cows had higher mean serum anti-J5 E coli IgG1 concentrations than did 3X cows. Additionally, 5XSR cows had higher mean serum anti-J5 E coli IgG1 concentrations than did 5XN cows. Vaccinated cows had higher mean serum anti-J5 E coli IgG2 concentrations than did control cows. The 5XN and 5XSR cows had higher mean serum anti-J5 E coli IgG2 concentrations than did 3X cows. The 5XSR cows had higher mean serum anti-J5 E coli IgG2 concentrations than did all other groups at 84 days after the fifth vaccination.
Sequential doses of core-antigen bacterins administered at different anatomic locations may improve antibody response in dairy cattle.
确定注射部位对J5大肠杆菌菌苗抗体反应的影响。
28头成年荷斯坦奶牛。
奶牛被随机分为对照组（n = 4头奶牛），不接种J5大肠杆菌菌苗；3X组（8头），在颈部左侧皮下注射3剂菌苗；5XN组（8头），在颈部左侧皮下注射5剂菌苗；或5XSR组（8头），依次在颈部左侧、颈部右侧、胸部右侧、胸部左侧和颈部左侧皮下注射5剂菌苗。采集奶牛血样以测定抗J5大肠杆菌IgG1和IgG2浓度。
接种疫苗的奶牛血清抗J5大肠杆菌IgG1平均浓度高于对照奶牛。5XN组和5XSR组奶牛血清抗J5大肠杆菌IgG1平均浓度高于3X组奶牛。此外，5XSR组奶牛血清抗J5大肠杆菌IgG1平均浓度高于5XN组奶牛。接种疫苗的奶牛血清抗J5大肠杆菌IgG2平均浓度高于对照奶牛。5XN组和5XSR组奶牛血清抗J5大肠杆菌IgG2平均浓度高于3X组奶牛。在第五次接种后84天时，5XSR组奶牛血清抗J5大肠杆菌IgG2平均浓度高于所有其他组。
在不同解剖部位依次接种核心抗原菌苗可能会提高奶牛的抗体反应。
Zotero 插件