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阿那曲唑、甲氨蝶呤和氟尿嘧啶对体外和体内子宫内膜异位症的抗增殖作用。

Antiproliferative effects of anastrozole, methotrexate, and 5-fluorouracil on endometriosis in vitro and in vivo.

机构信息

Faculté de Médecine, Université Paris Descartes, EA 1833, ERTi, AP-HP Hôpital Cochin, Paris, France.

出版信息

Fertil Steril. 2010 Oct;94(5):1632-8.e1. doi: 10.1016/j.fertnstert.2009.09.031. Epub 2010 Jan 4.

Abstract

OBJECTIVE

To investigate the effects of antiproliferative drugs (anastrozole, methotrexate, and 5-fluorouracil [5-FU]) on the proliferation of endometriotic cells in vitro and in vivo.

DESIGN

Ex vivo study on human endometrial and endometriotic cells in culture; establishment of a murine model using mice implanted with human endometriosis.

SETTING

University research center.

PATIENT(S): Ten patients with ovarian endometrioma, 10 patients with deep infiltrating endometriosis, and 10 patients without endometriosis.

INTERVENTION(S): Stromal and epithelial cells were extracted from endometrial and endometriotic biopsies from patients with endometriosis and from patients without endometriosis. Cells were treated in vitro with anastrozole, methotrexate, progesterone, or 5-FU. Human endometriotic lesions were implanted in nude mice. Mice were treated with 5-FU or phosphate-buffered saline during 4 weeks before sacrifice and extraction of the endometriotic implants.

MAIN OUTCOME MEASURE(S): Stromal and epithelial cell proliferation and pathology score of endometriotic implants.

RESULT(S): Although anastrozole, methotrexate, and progesterone were ineffective, 5-FU significantly decreased the proliferation of endometriotic cells in vitro and controlled the growth of both cells from ovarian endometrioma and deep infiltrating endometriosis.

CONCLUSION(S): Considering common features between endometriotic cells and tumor cells, the use of 5-FU could be an option in the management of severe endometriosis.

摘要

目的

研究抗增殖药物(阿那曲唑、甲氨蝶呤和 5-氟尿嘧啶[5-FU])对体外和体内子宫内膜异位细胞增殖的影响。

设计

对培养的人子宫内膜和子宫内膜异位细胞进行离体研究;建立一种将人子宫内膜异位症植入小鼠体内的小鼠模型。

地点

大学研究中心。

患者

10 例卵巢子宫内膜瘤患者,10 例深部浸润性子宫内膜异位症患者和 10 例无子宫内膜异位症患者。

干预措施

从子宫内膜异位症患者和无子宫内膜异位症患者的子宫内膜和子宫内膜异位症活检中提取基质和上皮细胞。将细胞在体外用阿那曲唑、甲氨蝶呤、孕酮或 5-FU 处理。将人子宫内膜异位病变植入裸鼠体内。在处死和提取子宫内膜异位植入物前的 4 周内,用 5-FU 或磷酸盐缓冲盐水处理小鼠。

主要观察指标

子宫内膜异位症植入物的基质和上皮细胞增殖及病理评分。

结果

尽管阿那曲唑、甲氨蝶呤和孕酮无效,但 5-FU 显著降低了体外子宫内膜异位细胞的增殖,并控制了来自卵巢子宫内膜瘤和深部浸润性子宫内膜异位症的细胞生长。

结论

考虑到子宫内膜异位细胞和肿瘤细胞之间的共同特征,5-FU 的使用可能是治疗严重子宫内膜异位症的一种选择。

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