Biomedical Sciences, School of Health and Social Science, Middlesex University, Hendon NW4 4BT, UK.
Metabolism. 2010 Aug;59(8):1145-55. doi: 10.1016/j.metabol.2009.09.037. Epub 2010 Jan 4.
This study investigated the biochemical relationship between human follicular/oocyte maturity and the levels of follicular fluid purines. Intrafollicular levels of purine metabolites and creatinine are associated with oocyte presence, and the presence of such high levels of adenosine indicates a privileged site with no adenosine deaminase activity. Subgrouping according to oocyte recovery and fertilization revealed differences in correlation between the purine metabolites: Only where an oocyte was recovered and subsequently fertilized did follicular fluid adenosine, adenine, and hypoxanthine levels correlate with each other. Significantly, purines' correlation with levels of the terminal degradation product, uric acid, could only be seen in failed fertilization samples. Given the established metabolic pathways for adenosine triphosphate/adenosine diphosphate/adenosine monophosphate degradation, the results indicate maximization of 2 purine salvage pathways (from adenine and hypoxanthine) that pivot on the presence of high adenosine levels. Such optimized recovery may be necessary to build a store of salvaged adenosine phosphate for oocyte survival.
本研究探讨了人类卵泡/卵母细胞成熟度与卵泡液嘌呤水平之间的生化关系。卵泡内嘌呤代谢物和肌酐的水平与卵母细胞的存在有关，而高水平的腺苷表明存在没有腺苷脱氨酶活性的特权部位。根据卵母细胞回收和受精情况进行亚组分析，发现嘌呤代谢物之间的相关性存在差异：只有在回收卵母细胞并随后受精的情况下，卵泡液中的腺苷、腺嘌呤和次黄嘌呤水平才相互关联。值得注意的是，只有在受精失败的样本中才能观察到嘌呤与终末降解产物尿酸水平的相关性。鉴于已建立的三磷酸腺苷/二磷酸腺苷/一磷酸腺苷降解代谢途径，研究结果表明，最大化了两条嘌呤补救途径（来自腺嘌呤和次黄嘌呤），这两条途径依赖于高水平的腺苷。这种优化的回收可能是为卵母细胞生存储存回收的腺苷磷酸盐所必需的。
