人乳头瘤病毒和 Epstein-Barr 病毒感染与宫颈腺癌患者年龄的关系。

The relationship between human papillomavirus and Epstein-Barr virus infections in relation to age of patients with cervical adenocarcinoma.

机构信息

Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan.

出版信息

Taiwan J Obstet Gynecol. 2009 Dec;48(4):370-4. doi: 10.1016/S1028-4559(09)60325-9.

DOI:10.1016/S1028-4559(09)60325-9

PMID:20045757

Abstract

OBJECTIVE

To examine the relationship between human papillomavirus (HPV) and Epstein-Barr virus (EBV) infections in relation to age of patients with cervical adenocarcinoma.

MATERIALS AND METHODS

Thirty samples of human cervical adenocarcinoma tissue were collected from the surgical pathology archive at Taipei Veterans General Hospital from 1996 to 2008. All samples were examined for EBV, HPV-16 and HPV-18 E6 DNA by conventional and real-time quantitative polymerase chain reaction assays.

RESULTS

HPV-16 DNA was detected in 10 cases (33.3%), HPV-18 DNA in 12 cases (40%), and EBV DNA in three cases (10%); there were negative findings in seven cases (23.3%). EBV combined with HPV-16 or HPV-18 was also detected in one case each. No link could be demonstrated between HPV and EBV in endocervical lesions. When 20 patients 45 years old were compared with 10 patients > 45 years old, HPV-18 E6 DNA was detected in 45% vs. 30% (9/20 vs. 3/10), HPV-16 E6 DNA in 40% vs. 20% (8/20 vs. 2/10), EBV DNA in 10% vs. 10% (2/20 vs. 1/10), and no virus DNA was detected in 10% vs. 50% (2/20 vs. 5/10). HPV and EBV were significantly more common in younger women (p < 0.001).

CONCLUSION

HPV-18 plays a major role in adenocarcinomas at any age. A high prevalence of HPV DNA is significantly associated with cervical adenocarcinoma, especially in younger women. The results do not support a role for EBV in cervical adenocarcinogenesis or any relationship between EBV and HPV infection in adenocarcinoma.

摘要

目的

探讨人乳头瘤病毒（HPV）和 Epstein-Barr 病毒（EBV）感染与宫颈腺癌患者年龄的关系。

材料和方法

本研究收集了 1996 年至 2008 年期间台北荣民总医院外科病理档案中的 30 例宫颈腺癌组织样本。所有样本均通过常规和实时定量聚合酶链反应检测 EBV、HPV-16 和 HPV-18 E6 DNA。

结果

10 例（33.3%）检测到 HPV-16 DNA，12 例（40%）检测到 HPV-18 DNA，3 例（10%）检测到 EBV DNA，7 例（23.3%）检测结果为阴性。还在 1 例中检测到 EBV 与 HPV-16 或 HPV-18 的联合感染。在宫颈内病变中，未发现 HPV 与 EBV 之间存在关联。与 45 岁以上的 10 例患者相比，20 例 45 岁以下的患者中 HPV-18 E6 DNA 的检出率为 45%（9/20）vs. 30%（3/10），HPV-16 E6 DNA 的检出率为 40%（8/20）vs. 20%（2/10），EBV DNA 的检出率为 10%（2/20）vs. 10%（1/10），而无病毒 DNA 的检出率为 10%（2/20）vs. 50%（5/10）。HPV 和 EBV 在年轻女性中更为常见（p<0.001）。