改良的哺乳动物细胞同步化方法提高了产量和同步化程度。
Modified method of mammalian cell synchronization improves yield and degree of synchronization.
作者信息
Cao G, Liu L M, Cleary S F
机构信息
Physiology Department, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
出版信息
Exp Cell Res. 1991 Apr;193(2):405-10. doi: 10.1016/0014-4827(91)90113-9.
PMID:2004653
Abstract
A modified method to synchronize CHO and HeLa cells is developed based upon a combined shaking-off and chemical blockage. This method has effectively blocked quiescent cells, which is the main obstacle of high degree synchronization. Flow-cytometry data show the improvement on the degree of synchronization and yield compared to two previously used methods.
摘要
基于振荡去除和化学阻断相结合的方法，开发了一种同步中国仓鼠卵巢（CHO）细胞和人宫颈癌细胞（HeLa）的改良方法。该方法有效阻断了静止细胞，而静止细胞是高度同步化的主要障碍。流式细胞术数据显示，与之前使用的两种方法相比，同步化程度和产量都有所提高。
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