Song Yumi, Song Yoon Joong, Ko Myung Kyoo
Department of Ophthalmology, Hanyang University Hospital, #17 Haengdang-dong, Seongdong-gu, Seoul, Korea.
Korean J Ophthalmol. 2009 Dec;23(4):296-300. doi: 10.3341/kjo.2009.23.4.296. Epub 2009 Dec 4.
This study was performed to examine the vascular network of the human iris using flat preparation.
The ciliary body-iris structures were separated from human eyeballs, and a portion of the irises were treated with trypsin to remove the pigment granules. These iris tissues were unfolded and placed onto glass slides using flat preparation, and the vascular network of each iris was examined by fluorescein microscopy. The ciliary body-iris structures separated from the remaining eyes were stained with hematoxylin-eosin without trypsin treatment and were examined by light microscopy.
The long posterior ciliary artery formed several branches before entering the iris root, and such branches formed the major arterial circle of the iris with diverse diameters in the vicinity of the iris root and the ciliary process. In the pupillary margin, the iris vasculature network formed a cone shape and then formed an arcade by connecting to adjacent vasculatures. In the vicinity of the collarette, the iris vasculature network formed the minor arterial circle of the iris with diverse diameters perpendicular to the arcade of the iris network located in the pupillary margin. In the pupillary margin, the capillaries were somewhat thick and connected to the irregular traveling iris vein.
The above findings explain the human iris vascular network and provide a theoretical basis for the sectoral filling of the iris vasculature seen in fluorescein iris angiography.
本研究旨在通过平铺制片法检查人虹膜的血管网络。
将睫状体 - 虹膜结构从人眼球分离，部分虹膜用胰蛋白酶处理以去除色素颗粒。这些虹膜组织经平铺制片展开并置于载玻片上，通过荧光素显微镜检查各虹膜的血管网络。将从其余眼球分离的睫状体 - 虹膜结构未经胰蛋白酶处理，用苏木精 - 伊红染色，通过光学显微镜检查。
长后睫状动脉在进入虹膜根部前形成多个分支，这些分支在虹膜根部和睫状体附近形成直径各异的虹膜大动脉环。在瞳孔边缘，虹膜血管网络呈锥形，然后通过与相邻血管相连形成弓状结构。在瞳孔缘附近，虹膜血管网络形成与位于瞳孔边缘的虹膜网络弓状结构垂直、直径各异的虹膜小动脉环。在瞳孔边缘，毛细血管稍粗，与走行不规则的虹膜静脉相连。
上述发现解释了人虹膜血管网络，为荧光素虹膜血管造影中所见的虹膜血管扇形充盈提供了理论基础。
