Nishimura Kazuhiko, Yanase Takako, Nakagawa Hiroshi, Matsuo Saburo, Ohnishi Yoshihiro, Yamasaki Shinji
Laboratory of Infectious Diseases Control, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
J Parasitol. 2009 Aug;95(4):781-6. doi: 10.1645/GE-1883.1.
Polyamines are essential for proliferation of Trypanosoma brucei brucei, and feeding rats polyamine-deficient chow (PDC) decreases their blood polyamine concentrations. Proliferation of T. b. brucei (IL-tat 1.4 strain) (IL) is not restrained within PDC-fed rats. However, symptoms of IL-infected rats such as anemia decrease by PDC feeding. We reported cytokine and nitric oxide (NO) production of T. b. gambiense (Wellcome strain [WS])-infected rats were affected by PDC feeding, and WS proliferation was restrained. Therefore, we investigated whether the change in production of cytokines and NO by PDC feeding affects IL proliferation and decreases symptoms in vivo. In IL-infected PDC-fed rats, NO, interleukin (IL)-12, and tumor necrosis factor-alpha production increased while interferon-gamma and IL-10 decreased compared to normal chow-fed rats. IL proliferation was restrained by NO production when it was co-cultured with spleen cells harvested from uninfected rats. In contrast, IL proliferation in infected rats was not changed by PDC feeding, although NO production was increased. The results suggest that changes in cytokines and NO production in IL-infected rats by PDC feeding have little influence on IL proliferation. However, they may serve to decrease symptoms.
多胺对于布氏布氏锥虫的增殖至关重要，给大鼠喂食缺乏多胺的饲料（PDC）会降低其血液中的多胺浓度。布氏布氏锥虫（IL-tat 1.4株）（IL）在喂食PDC的大鼠体内的增殖并未受到抑制。然而，喂食PDC可减轻IL感染大鼠的贫血等症状。我们报道了喂食PDC会影响冈比亚锥虫（威康株[WS]）感染大鼠的细胞因子和一氧化氮（NO）产生，并且WS的增殖受到抑制。因此，我们研究了喂食PDC导致的细胞因子和NO产生变化是否会影响IL的增殖并减轻体内症状。与喂食正常饲料的大鼠相比，在IL感染且喂食PDC的大鼠中，NO、白细胞介素（IL）-12和肿瘤坏死因子-α的产生增加，而干扰素-γ和IL-10减少。当IL与从未感染大鼠收获的脾细胞共培养时，其增殖受到NO产生的抑制。相比之下，尽管NO产生增加，但喂食PDC对感染大鼠中IL的增殖没有影响。结果表明，喂食PDC导致IL感染大鼠中细胞因子和NO产生的变化对IL增殖影响不大。然而，它们可能有助于减轻症状。
