不同方法测定聚乙二醇与聚（酸酐）纳米粒子的结合程度。
Different approaches for determination of the attachment degree of polyethylene glycols to poly(anhydride) nanoparticles.
机构信息
Department of Pharmaceutical Technology, Faculty of Pharmacy, Sofia, Bulgaria.
出版信息
Drug Dev Ind Pharm. 2010 Jun;36(6):676-80. doi: 10.3109/03639040903443434.
PURPOSE
The aim of the study was to find an appropriate method for determination of the attachment degree of polyethylene glycol (PEG) to poly(anhydride) nanoparticles.
METHODS
The nanoparticles were modified with hydroxy-functionalized or with amino-functionalized PEGs. Three methods for their determination were applied and examined: in particular colorimetry, nuclear magnetic resonance ( (1) H-NMR), and elemental analysis (EA).
RESULTS
The attachment degrees determined by (1) H-NMR and colorimetry were similar. The associated amounts of PEGs estimated on the basis of EA differed significantly than those determined by colorimetry and (1) H-NMR.
CONCLUSION
The colorimetric determination was considered fast and simple technique, but (1) H-NMR contributed to more precise results.
目的
本研究旨在寻找一种合适的方法来测定聚乙二醇（PEG）与聚（酸酐）纳米粒子的结合程度。
方法
将纳米粒子用羟基化或氨基化的 PEG 进行修饰。应用并考察了三种测定方法：比色法、核磁共振（1H-NMR）和元素分析（EA）。
结果
（1）H-NMR 和比色法测定的结合度相似。基于 EA 估算的 PEG 量与比色法和（1）H-NMR 测定的结果有显著差异。
结论
比色法测定被认为是一种快速简单的技术，但（1）H-NMR 有助于获得更精确的结果。
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