Université de Lyon, UPSP 2007.03.135, Lyon Cedex 08, France.
Biomaterials. 2010 Mar;31(8):2043-54. doi: 10.1016/j.biomaterials.2009.11.107. Epub 2010 Jan 6.
In order to improve the reliability and the mechanical properties of orthopaedic hip prosthesis, new ceramic composites starting with nanosized powders of alumina and zirconia have been recently developed. The aim of the present study was to investigate the biological tolerance of one of these sintered ceramics and of its alumina and zirconia constitutive nanosized powders with both in vitro and in vivo approaches. At first, osteoblasts and fibroblasts were cultured either upon sintered ceramic discs with polished or rough surfaces or in the presence of the corresponding alumina or zirconia powders at various concentrations. Thereafter, we chronically injected these powders in the knee articulation of rats. In vitro, the materials showed no deleterious effect on cell proliferation, extra-cellular matrix production (human type I collagen and fibronectin) or on cell morphology. In vivo, the histological examination showed only a very moderate and non-specific granulomatous response of the synovial membrane but no major inflammation as clinically described with metals or polyethylene wear debris. Besides its improved physical properties, this recently developed alumina-zirconia composite showed satisfactory biocompatibility.
为了提高骨科髋关节假体的可靠性和机械性能，最近开发了一种新型陶瓷复合材料，其起始粉末为纳米级氧化铝和氧化锆。本研究的目的是通过体外和体内方法研究这种烧结陶瓷及其氧化铝和氧化锆组成的纳米粉末的生物耐受性。首先，将成骨细胞和纤维母细胞培养在经过抛光或粗糙处理的烧结陶瓷盘上，或者在不同浓度的相应氧化铝或氧化锆粉末存在的情况下进行培养。然后，我们将这些粉末长期注射到大鼠膝关节中。体外实验结果表明，这些材料对细胞增殖、细胞外基质（人 I 型胶原和纤维连接蛋白）的产生或细胞形态没有不良影响。体内实验结果表明，组织学检查仅显示滑膜有非常轻微和非特异性的肉芽组织反应，但没有像临床上描述的金属或聚乙烯磨损颗粒那样的严重炎症。除了改善物理性能外，这种新型氧化铝-氧化锆复合材料还具有良好的生物相容性。
