Ⅱ型和Ⅲ型味蕾细胞在大鼠中优先表达红藻氨酸谷氨酸受体。
Type II and III Taste Bud Cells Preferentially Expressed Kainate Glutamate Receptors in Rats.
机构信息
Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, Korea.
出版信息
Korean J Physiol Pharmacol. 2009 Dec;13(6):455-60. doi: 10.4196/kjpp.2009.13.6.455. Epub 2009 Dec 31.
Glutamate-induced cobalt uptake reveals that non-NMDA glutamate receptors (GluRs) are present in rat taste bud cells. Previous studies involving glutamate induced cobalt staining suggest this uptake mainly occurs via kainate type GluRs. It is not known which of the 4 types of taste bud cells express subunits of kainate GluR. Circumvallate and foliate papillae of Sprague-Dawley rats (45~60 days old) were used to search for the mRNAs of subunits of non-NMDA GluRs using RT-PCR with specific primers for GluR1-7, KA1 and KA2. We also performed RT-PCR for GluR5, KA1, PLCbeta2, and NCAM/SNAP 25 in isolated single cells from taste buds. Taste epithelium, including circumvallate or foliate papilla, express mRNAs of GluR5 and KA1. However, non-taste tongue epithelium expresses no subunits of non-NMDA GluRs. Isolated single cell RT-PCR reveals that the mRNAs of GluR5 and KA1 are preferentially expressed in Type II and Type III cells over Type I cells.
谷氨酸诱导的钴摄取表明非 NMDA 谷氨酸受体（GluR）存在于大鼠味蕾细胞中。先前涉及谷氨酸诱导的钴染色的研究表明，这种摄取主要通过 kainate 型 GluR 发生。目前尚不清楚哪种类型的味蕾细胞表达 kainate GluR 的亚基。使用针对 GluR1-7、KA1 和 KA2 的特异性引物，通过 RT-PCR，我们使用 Sprague-Dawley 大鼠（45~60 天龄）的环状和叶片乳头来搜索非 NMDA GluR 亚基的 mRNA。我们还在味蕾的分离单细胞中进行了 GluR5、KA1、PLCβ2 和 NCAM/SNAP 25 的 RT-PCR。味觉上皮，包括环状乳头或叶片乳头，表达 GluR5 和 KA1 的 mRNA。然而，非味觉舌上皮不表达非 NMDA GluR 的亚基。分离的单细胞 RT-PCR 表明，GluR5 和 KA1 的 mRNA 优先在 II 型和 III 型细胞中表达，而不是在 I 型细胞中表达。
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