亲和选择-超滤/HPLC-MS研究进展

[Advances in the study of affinity selection-ultrafiltration/HPLC-MS].

作者信息

Zhao Hong-Yan, Zhang Yong-Zhong, Xiao Chun-Ling

机构信息

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.

出版信息

Yao Xue Xue Bao. 2009 Oct;44(10):1084-8.

PMID:20055128

Abstract

Affinity selection-ultrafiltration/HPLC-MS is the combination of the ultrafiltration and HPLC-MS, mainly used for screening small active molecular substances from combinatorial libraries and natural product extracts, which can bind to solution-phase targets. Besides, it can be used in metabolic screening and characterization of ligand-receptor binding. It is a complement to the traditional methods of screening and identifying drugs. This review describes its principle and application in drug study.

摘要

亲和选择超滤/高效液相色谱-质谱联用技术是超滤与高效液相色谱-质谱联用的技术，主要用于从组合文库和天然产物提取物中筛选能够与溶液相靶点结合的小分子活性物质。此外，它还可用于配体-受体结合的代谢筛选和表征。它是对传统药物筛选和鉴定方法的一种补充。本文综述了其原理及其在药物研究中的应用。