Wang Qing-Hao, Zhang Bo-Li
Postdoctoral Station, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Yao Xue Xue Bao. 2009 Aug;44(8):927-30.
The molecular maker system TRAP was utilized to develop a novel more accurate tool to identify the variety and establish the evolutionary relationship of different kind of Salvia miltiorrhiza Bge. PopGene 32 software was applied to conduct the cluster analysis and genetic dendrogram establishment. The results showed that 203 different fragments were amplified with 6 pair primers using the TRAP marker system. The polymorphic fragments number is 43, which takes up to 21.1%. The cluster analysis shows that the 4 materials we used in this study can be classified into 2 main groups and 3 subgroups. The genetic identity is 0.0788 and the average genetic distance is 0.9245 among the four materials in this study. A new tool using the TRAP marker system is more accurate and can be used to identify different kind of Salvia miltiorrhiza Bge at molecular level.
利用分子标记系统TRAP开发了一种新型更精确的工具,用于鉴定不同种类丹参的品种并建立其进化关系。应用PopGene 32软件进行聚类分析和构建遗传树状图。结果表明,使用TRAP标记系统,6对引物扩增出203个不同片段。多态性片段数为43个,占21.1%。聚类分析表明,本研究中使用的4个材料可分为2个主要类群和3个亚类群。本研究中4个材料之间的遗传一致性为0.0788,平均遗传距离为0.9245。使用TRAP标记系统的新工具更准确,可用于在分子水平上鉴定不同种类的丹参。