Filipenko E A, Deĭneko E V, Shumnyĭ V K
Genetika. 2009 Nov;45(11):1461-75.
Experimental data from analysis of exogenous DNA (T-DNA) insertion sites in transgenic plants are summarized. Arguments are considered in favor and against the existence of genome DNA regions preferred for transgene integration that are determined by distinctive features characterizing the organization and nucleotide composition of the plant genome and the structure and conformational state of the chromatin. The main stages of T-DNA integration into a plant chromosome and possible molecular mechanisms of this process are discussed.
总结了转基因植物中外源DNA(T-DNA)插入位点分析的实验数据。讨论了支持和反对存在转基因整合偏好的基因组DNA区域的观点,这些区域由植物基因组的组织和核苷酸组成以及染色质的结构和构象状态的独特特征所决定。讨论了T-DNA整合到植物染色体中的主要阶段以及该过程可能的分子机制。