Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, USA.
J Am Chem Soc. 2010 Feb 3;132(4):1252-4. doi: 10.1021/ja908890q.
Water-soluble, cationic conjugated polymer binds single-stranded DNA with higher affinity than it binds double-stranded or otherwise "folded" DNA. This stronger binding results from the greater hydrophobicity of single-stranded DNA. Upon reducing the strength of the hydrophobic interactions, the electrostatic attraction becomes the important interaction that regulates the binding between the water-soluble conjugated polymer and DNA. The different affinities between the cationic conjugated polymer and various forms of DNA (molecular beacons and its open state; single-stranded DNA and double-stranded DNA and single-stranded DNA and complex DNA folds) can be used to design a variety of biosensors.
水溶性阳离子共轭聚合物与单链 DNA 的结合亲和力高于双链或其他“折叠”DNA。这种更强的结合源于单链 DNA 的更大疏水性。当降低疏水力的强度时，静电吸引成为调节水溶性共轭聚合物与 DNA 之间结合的重要相互作用。阳离子共轭聚合物与各种形式的 DNA（分子信标及其开放状态；单链 DNA 和双链 DNA 以及单链 DNA 和复杂 DNA 折叠）之间的不同亲和力可用于设计各种生物传感器。
