关于阳离子型、水溶性共轭聚合物与 DNA 的结合：静电相互作用和疏水相互作用。
On the binding of cationic, water-soluble conjugated polymers to DNA: electrostatic and hydrophobic interactions.
机构信息
Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, USA.
出版信息
J Am Chem Soc. 2010 Feb 3;132(4):1252-4. doi: 10.1021/ja908890q.
Abstract
Water-soluble, cationic conjugated polymer binds single-stranded DNA with higher affinity than it binds double-stranded or otherwise "folded" DNA. This stronger binding results from the greater hydrophobicity of single-stranded DNA. Upon reducing the strength of the hydrophobic interactions, the electrostatic attraction becomes the important interaction that regulates the binding between the water-soluble conjugated polymer and DNA. The different affinities between the cationic conjugated polymer and various forms of DNA (molecular beacons and its open state; single-stranded DNA and double-stranded DNA and single-stranded DNA and complex DNA folds) can be used to design a variety of biosensors.
摘要
水溶性阳离子共轭聚合物与单链 DNA 的结合亲和力高于双链或其他“折叠”DNA。这种更强的结合源于单链 DNA 的更大疏水性。当降低疏水力的强度时，静电吸引成为调节水溶性共轭聚合物与 DNA 之间结合的重要相互作用。阳离子共轭聚合物与各种形式的 DNA（分子信标及其开放状态；单链 DNA 和双链 DNA 以及单链 DNA 和复杂 DNA 折叠）之间的不同亲和力可用于设计各种生物传感器。
相似文献
1
On the binding of cationic, water-soluble conjugated polymers to DNA: electrostatic and hydrophobic interactions.关于阳离子型、水溶性共轭聚合物与 DNA 的结合：静电相互作用和疏水相互作用。
J Am Chem Soc. 2010 Feb 3;132(4):1252-4. doi: 10.1021/ja908890q.
2
Tuning backbones and side-chains of cationic conjugated polymers for optical signal amplification of fluorescent DNA detection.调整阳离子共轭聚合物的主链和侧链以实现荧光DNA检测的光信号放大
Biosens Bioelectron. 2009 Jun 15;24(10):2973-8. doi: 10.1016/j.bios.2009.03.003. Epub 2009 Mar 16.
3
DNA hybridization detection with water-soluble conjugated polymers and chromophore-labeled single-stranded DNA.利用水溶性共轭聚合物和发色团标记的单链DNA进行DNA杂交检测。
J Am Chem Soc. 2003 Jan 29;125(4):896-900. doi: 10.1021/ja027152+.
4
Cationic salt-responsive bottle-brush polymers.阳离子盐响应性瓶刷聚合物。
Macromol Rapid Commun. 2013 Apr 25;34(8):645-51. doi: 10.1002/marc.201300088. Epub 2013 Mar 13.
5
Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: the role of electrostatic and hydrophobic interactions.使用水溶性共轭聚合物进行DNA序列检测中的时间分辨能量转移：静电和疏水相互作用的作用
Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11634-9. doi: 10.1073/pnas.0404574101. Epub 2004 Jul 28.
6
Single stranded siRNA complexation through non-electrostatic interactions.单链 siRNA 通过非静电相互作用进行复合物形成。
Biomaterials. 2017 Jan;113:230-242. doi: 10.1016/j.biomaterials.2016.10.035. Epub 2016 Oct 29.
7
Fluorescent amplifying recognition for DNA G-quadruplex folding with a cationic conjugated polymer: a platform for homogeneous potassium detection.阳离子共轭聚合物对DNA G-四链体折叠的荧光放大识别：一种均相钾检测平台
J Am Chem Soc. 2005 Sep 7;127(35):12343-6. doi: 10.1021/ja051507i.
8
Interactions in DNA Condensation: An Important Factor for Improving the Efficacy of Gene Transfection.DNA 凝聚中的相互作用：提高基因转染效果的重要因素。
Bioconjug Chem. 2019 Feb 20;30(2):284-292. doi: 10.1021/acs.bioconjchem.8b00805. Epub 2018 Dec 21.
9
Responsive polymer gels: double-stranded versus single-stranded DNA.响应性聚合物凝胶：双链DNA与单链DNA
J Phys Chem B. 2007 Sep 20;111(37):10886-96. doi: 10.1021/jp0713108. Epub 2007 Aug 30.
10
DNA-assisted photoinduced charge transfer between a cationic poly(phenylene vinylene) and a cationic fullerene.阳离子聚对苯撑乙烯与阳离子富勒烯之间的DNA辅助光诱导电荷转移。
Phys Chem Chem Phys. 2015 Jun 28;17(24):15675-8. doi: 10.1039/c5cp01309g. Epub 2015 Apr 20.
引用本文的文献
1
DNA Framework-Engineered Assembly of Cyanine Dyes for Structural Identification of Nucleic Acids.用于核酸结构鉴定的花菁染料的DNA框架工程组装
JACS Au. 2024 Feb 22;4(3):1125-1133. doi: 10.1021/jacsau.3c00826. eCollection 2024 Mar 25.
2
Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids.疏水相互作用：核酸生物医学应用中一种有前景的驱动力。
Adv Sci (Weinh). 2020 Jul 1;7(16):2001048. doi: 10.1002/advs.202001048. eCollection 2020 Aug.
3
Polythiophenes with Cationic Phosphonium Groups as Vectors for Imaging, siRNA Delivery, and Photodynamic Therapy.带有阳离子鏻基团的聚噻吩作为成像、siRNA递送和光动力疗法的载体
Nanomaterials (Basel). 2020 Jul 22;10(8):1432. doi: 10.3390/nano10081432.
4
Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays.决定分子信标适配体/共轭聚电解质生物测定中检测范围扩展的主要因素。
Chem Sci. 2015 Mar 1;6(3):1887-1894. doi: 10.1039/c4sc03258f. Epub 2015 Jan 7.
5
Utilizing Intrinsic Properties of Polyaniline to Detect Nucleic Acid Hybridization through UV-Enhanced Electrostatic Interaction.利用聚苯胺的固有特性通过紫外线增强的静电相互作用检测核酸杂交。
Biomacromolecules. 2015 Oct 12;16(10):3217-25. doi: 10.1021/acs.biomac.5b00935. Epub 2015 Sep 29.
6
A novel property of DNA - as a bioflotation reagent in mineral processing.DNA 的新特性 - 作为矿物加工中的生物浮选试剂。
PLoS One. 2012;7(7):e39316. doi: 10.1371/journal.pone.0039316. Epub 2012 Jul 2.
7
Fluorescence protection assay: a novel homogeneous assay platform toward development of aptamer sensors for protein detection.荧光保护测定法：一种新型的均相测定平台，用于开发用于蛋白质检测的适体传感器。
Nucleic Acids Res. 2011 Oct;39(18):e122. doi: 10.1093/nar/gkr559. Epub 2011 Jul 8.
8
Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes.使用未经修饰的金纳米粒子和共轭聚电解质进行 DNA、小分子、蛋白质和离子的比色检测。
Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10837-41. doi: 10.1073/pnas.1005632107. Epub 2010 Jun 1.
本文引用的文献
1
Colorimetric and fluorometric detection of nucleic acids using cationic polythiophene derivatives.使用阳离子聚噻吩衍生物对核酸进行比色和荧光检测。
Angew Chem Int Ed Engl. 2002 May 3;41(9):1548-51. doi: 10.1002/1521-3773(20020503)41:9<1548::aid-anie1548>3.0.co;2-i.
2
Thermodynamic basis for the optimization of binding-induced biomolecular switches and structure-switching biosensors.结合诱导的生物分子开关和结构转换生物传感器优化的热力学基础。
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13802-7. doi: 10.1073/pnas.0904005106. Epub 2009 Aug 5.
3
High specificity, electrochemical sandwich assays based on single aptamer sequences and suitable for the direct detection of small-molecule targets in blood and other complex matrices.高特异性的基于单适配体序列的电化学夹心分析方法，适用于直接检测血液和其他复杂基质中的小分子靶标。
J Am Chem Soc. 2009 May 27;131(20):6944-5. doi: 10.1021/ja901315w.
4
Use of sloppy molecular beacon probes for identification of mycobacterial species.使用不精确的分子信标探针鉴定分枝杆菌菌种。
J Clin Microbiol. 2009 Apr;47(4):1190-8. doi: 10.1128/JCM.02043-08. Epub 2009 Jan 26.
5
Optimizing the cationic conjugated polymer-sensitized fluorescent signal of dye labeled oligonucleotide for biosensor applications.优化用于生物传感器应用的染料标记寡核苷酸的阳离子共轭聚合物敏化荧光信号。
Biosens Bioelectron. 2009 Jan 1;24(5):1067-73. doi: 10.1016/j.bios.2008.07.029. Epub 2008 Jul 26.
6
Fluorescent conjugated polyelectrolyte as an indicator for convenient detection of DNA methylation.荧光共轭聚电解质作为便捷检测DNA甲基化的指示剂。
J Am Chem Soc. 2008 Aug 27;130(34):11338-43. doi: 10.1021/ja8011963. Epub 2008 Aug 1.
7
Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures.利用金纳米颗粒和合理设计的适体结构进行可卡因可视化检测。
Small. 2008 Aug;4(8):1196-200. doi: 10.1002/smll.200800057.
8
Application of cationic conjugated polymers in microarrays using label-free DNA targets.阳离子共轭聚合物在使用无标记DNA靶标的微阵列中的应用。
Nat Protoc. 2007;2(9):2148-51. doi: 10.1038/nprot.2007.307.
9
Ion motion in conjugated polyelectrolyte electron transporting layers.共轭聚电解质电子传输层中的离子运动。
J Am Chem Soc. 2007 Sep 12;129(36):10976-7. doi: 10.1021/ja072612q. Epub 2007 Aug 17.
10
A sensitive and homogeneous SNP detection using cationic conjugated polymers.使用阳离子共轭聚合物进行灵敏且均相的单核苷酸多态性检测。
J Am Chem Soc. 2007 Apr 11;129(14):4154-5. doi: 10.1021/ja070452c. Epub 2007 Mar 20.
Zotero 插件