Department of Periodontology, Institute of Clinical Dentistry, University of Tromsø, N-9037 Breivika, Norway.
J Periodontol. 2010 Jan;81(1):52-61. doi: 10.1902/jop.2009.090315.
We previously reported a high prevalence of a combination of alleles 2 at positions interleukin (IL)-1A(-889) and IL-1B(+3954) in an Arabic population with gingivitis. In a steady state, the proportion of sites with bleeding on probing (BOP) was consistently reduced. The aim of the present study is to expand this observation by considering the site level.
Fifty healthy non-smoking volunteers, 19 to 28 years of age, participated in this study. Clinical examinations included probing depth, BOP, plaque index (PI), and calculus. Examinations were repeated after 2 and 4 weeks, and subjects were advised not to change oral hygiene habits. Polymorphisms in the IL-1 gene cluster were assessed using a reverse hybridization assay.
Twenty-six subjects (52%) carried alleles 2 at positions IL-1A(-889) and IL-1B(+3954) and were designated genotype positive. In multivariate multilevel models of BOP, bleeding tendency in subjects with positive IL-1 genotype was only reduced at sites with PI 0 (odds ratio of 0.98, 0.65, and 0.56 at baseline and 2 and 4 weeks, respectively; chi(2)((3)) = 11.946; P = 0.008) or 1 (chi(2)((3)) = 6.027; P = 0.110). A decreased bleeding tendency at certain tooth types in subjects who were IL-1 positive was largely related to the relative cleanliness of these areas. Random parts of the models revealed very low biserial correlations of 0.13 to 0.15 at the site level, whereas correlations were considerably higher (0.76 to 0.83) at the subject level.
Multilevel modeling of site-specific data yielded new information about the influence of the IL-1 genotype in plaque-induced gingivitis.
我们之前报道了在患有牙龈炎的阿拉伯人群中，白细胞介素（IL）-1A(-889)和 IL-1B(+3954)位置的等位基因 2 的组合的高患病率。在稳定状态下，探测时出血（BOP）的部位比例持续降低。本研究的目的是通过考虑位点水平来扩展这一观察结果。
50 名年龄在 19 至 28 岁之间的非吸烟健康志愿者参加了这项研究。临床检查包括探诊深度、BOP、菌斑指数（PI）和牙石。检查在 2 周和 4 周后重复进行，并且告知受试者不要改变口腔卫生习惯。使用反向杂交测定法评估 IL-1 基因簇中的多态性。
26 名受试者（52%）携带 IL-1A(-889)和 IL-1B(+3954)位置的等位基因 2，被指定为阳性基因型。在 BOP 的多变量多层次模型中，具有阳性 IL-1 基因型的受试者的出血倾向仅在 PI 为 0（优势比分别为 0.98、0.65 和 0.56，基线和 2 周和 4 周时；chi(2)((3)) = 11.946；P = 0.008）或 1（chi(2)((3)) = 6.027；P = 0.110）的位点减少。在 IL-1 阳性的受试者中，某些牙齿类型出血倾向降低主要与这些区域的相对清洁度有关。模型的随机部分显示，在站点水平上的双序列相关性非常低，为 0.13 至 0.15，而在受试者水平上的相关性要高得多（0.76 至 0.83）。
对特定于站点的数据进行多层次建模提供了有关 IL-1 基因型在菌斑诱导的牙龈炎中的影响的新信息。
