Peconick Ana P, Sossai Sidimar, Medeiros Carla L, Carvalho Gabriel D, Vargas Marlene I, Patarroyo Joaquín H
Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Rev Bras Parasitol Vet. 2008 Sep;17 Suppl 1:18-23.
The vaccination represents optimum method evaluated with effective cost to prevent economic losses and to increase the duration and quality of life of the production animals. . Diverse vaccines are produced from the intestinal protein Bm86 of the Rhipicephalus. (B.) microplus. The knowledge of the conservation of the gene bm86 is very important to evaluate the vaccine efficiency and the possibility of reaction crossed between different species of ticks. Samples of R. (B.) microplus come from different localities had been sequenced. The analyses of multiple alignments of the sequences had been made through the BioEdit program 7.0.5.3 version and the verification of polymorphism for visual inspection. In this work the alignment of all was become fulfilled sequences using itself BLAST in the search for similarity. Similarity was observed enters the sequenced fragments of R. (B.) microplus with the sequence of the protein Rs86 de Rhipicephalus sanguineus and with protein HA98 of the tick Hyalomma anatolicum anatolicum. The results give molecular support to synthetic the vaccine use based in the gene bm86 (SBm7462®) to be used in different species of ticks.
疫苗接种是经成本效益评估的最佳方法，可预防经济损失并延长生产动物的寿命、提高其生活质量。多种疫苗由微小牛蜱肠道蛋白Bm86制备而成。了解bm86基因的保守性对于评估疫苗效率以及不同蜱种之间交叉反应的可能性非常重要。已对来自不同地区的微小牛蜱样本进行了测序。通过BioEdit 7.0.5.3版本程序进行了序列的多序列比对分析，并通过目视检查验证多态性。在本研究中，使用BLAST自身对所有已完成测序的序列进行比对以寻找相似性。观察到微小牛蜱的测序片段与血红扇头蜱的Rs86蛋白序列以及小亚璃眼蜱的HA98蛋白之间存在相似性。这些结果为基于bm86基因（SBm7462®）合成用于不同蜱种的疫苗提供了分子支持。
