Shigueru Flavio Jojima, Garcia João Luís, Vidotto Marilda Carlos, Balarin Mara Regina S, Fabretti Andrei Kleiton, Gasparini Marcela R, Coelho Adriana Letícia M, Vidotto Odilon
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina,Campus Universitário, Londrina, PR 86051-990, Brazil.
Rev Bras Parasitol Vet. 2008 Sep;17 Suppl 1:277-83.
Canine babesiosis is a worldwide disease caused by the protozoan of Babesia genus. Babesia canis and B. gibsoni are both species that naturally infect dogs. The objective of this study was to evaluate the infection of Babesia species in dogs attended at the Londrina State University Veterinary Teaching Hospital (HV-UEL). It was selected 282 dogs seen at the Londrina State University Veterinary Teaching Hospital (HV-UEL) between April of 2005 and May of 2006. They presented anemia (Packed Cell Volume<25%), thrombocytopenia (Platelet count <150000/mm3), leukopenia (White blood cell count<5000/mm3) or a combination of two or three of these alterations at the moment of the consultation. The presence of Babesia sp was determined by the amplification of a specific fragment of DNA of the Babesia genus by PCR. Microscopic examination of Giemsa-stained blood smears detected 38 (13.5%) positive samples against 105 identified by PCR from 282 dogs. The positive samples were submitted to PCR-RFLP by Hinf I that allows distinguishing the species of B. canis vogeli and B. gibsoni. From 282 dogs, Babesia sp infection was identified in 105 (37.2%). From these 105 positive samples, the PCR-RFLP identified 66 (23.4%) samples with a profile compatible to B. canis vogeli and 39 (13, 8%) to B. gibsoni. As conclusions, the results obtained allow to affirm that the babesiose is an important differential for dogs that present anemia, leukopenia and thrombocytopenia and, B. canis vogeli is the subspecies that is present in the most of the cases of babesiose in the population of dogs studied and, that B. gibsoni is also present causing babesiosis in dogs of the Londrina region, Parana State, Brazil.
犬巴贝斯虫病是一种由巴贝斯属原生动物引起的全球性疾病。犬巴贝斯虫和吉氏巴贝斯虫都是自然感染犬类的物种。本研究的目的是评估在隆德里纳州立大学兽医教学医院（HV-UEL）就诊的犬类中巴贝斯虫物种的感染情况。选取了2005年4月至2006年5月期间在隆德里纳州立大学兽医教学医院（HV-UEL）就诊的282只犬。它们在就诊时出现贫血（红细胞压积<25%）、血小板减少（血小板计数<150000/mm3）、白细胞减少（白细胞计数<5000/mm3）或这些改变中的两种或三种的组合。通过PCR扩增巴贝斯属DNA的特定片段来确定巴贝斯虫属的存在。对吉姆萨染色的血涂片进行显微镜检查，在282只犬中检测到38份（13.5%）阳性样本，而通过PCR鉴定出105份阳性样本。将阳性样本进行Hinf I酶切的PCR-RFLP分析，以区分犬巴贝斯虫沃氏亚种和吉氏巴贝斯虫。在282只犬中，鉴定出105只（37.2%）感染了巴贝斯虫属。在这105份阳性样本中，PCR-RFLP分析鉴定出66份（23.4%）样本的图谱与犬巴贝斯虫沃氏亚种相符，39份（13.8%）与吉氏巴贝斯虫相符。作为结论，所获得的结果可以肯定，巴贝斯虫病是出现贫血、白细胞减少和血小板减少的犬类的重要鉴别诊断疾病，并且犬巴贝斯虫沃氏亚种是在所研究的犬类群体中大多数巴贝斯虫病病例中存在的亚种，而且吉氏巴贝斯虫也存在于巴西巴拉那州隆德里纳地区的犬类中，引起犬巴贝斯虫病。
