Cardozo Sergian V, Teixeira Filho Walter L, Lopes Carlos Wilson G
Departamento de Parasitologia Animal, Instituto de Veterinária, Hospital Universitário Antônio Pedro, Centro de Ciências Médicas, Universidade Federal Fluminense, 303 Centro, Niterói, RJ 24030-210, Brazil.
Rev Bras Parasitol Vet. 2008 Sep;17 Suppl 1:351-3.
The objective of this work is to evaluate different diagnostic techniques for Cryptosporidium baileyi oocysts in broiler chickens fecal samples infected naturally. So 90 samples were collected and conditioned in bottles with 10% formaline and submitted to Sheater s technique in bright field. Besides it, duplicate smears of the same samples were stained by safranin-methylene blue and modified Ziehl-Neelsen. Among the staining techniques, modified Ziehl-Neelsen got better result in the detention of C. baileyi oocysts in comparison to safranin-methylene blue. In spite of no significant differences among these techniques in the diagnosis of C. baileyi oocysts of fecal samples, bright field presented better results.
这项工作的目的是评估自然感染隐孢子虫的肉鸡粪便样本中贝利隐孢子虫卵囊的不同诊断技术。因此,收集了90份样本,置于含有10%甲醛的瓶中处理,并在明场下采用Sheater技术进行检测。此外,对相同样本的涂片进行番红-亚甲蓝和改良齐-尼氏染色。在这些染色技术中,与番红-亚甲蓝相比,改良齐-尼氏染色在检测贝利隐孢子虫卵囊方面取得了更好的结果。尽管在粪便样本中贝利隐孢子虫卵囊的诊断中这些技术之间没有显著差异,但明场检测呈现出更好的结果。