Institute of Marine Biology, National Taiwan Ocean University, 2-Peining Rd, Keelung, Taiwan.
J Hazard Mater. 2010 May 15;177(1-3):281-9. doi: 10.1016/j.jhazmat.2009.12.030. Epub 2010 Jan 8.
This work demonstrated that Shewanella decolorationis NTOU1 decolorized 200 mg l(-1) of crystal violet, malachite green, or methyl violet B within 2-11h under anaerobic conditions at 35 degrees C. The initial color removal rate of malachite green was highest, while that of methyl violet was lowest. GC/MS analyses of the intermediate compounds produced during and after decolorization of malachite green and methyl violet B suggested that biodegradation of these dyes involved reduction to leuco form, N-demethylation, and reductive splitting of the triphenyl rings. The number of N-methylated groups of these dyes might have influenced decolorization rates and the reductive splitting of the triphenyl rings of these dyes. Cytotoxicity and antimicrobial test data showed that malachite green and methyl violet B solution (100 mg l(-1)) were toxic. Toxicity of the dyes decreased after their decolorization, but further incubation resulted in increased toxicity.
该工作表明，希瓦氏菌（Shewanella decolorationis）NT0U1 在 35°C 厌氧条件下，2-11 小时内可将 200mg/L 的结晶紫、孔雀石绿或甲基紫 B 脱除颜色。孔雀石绿的初始脱色率最高，而甲基紫的初始脱色率最低。GC/MS 分析在脱除孔雀石绿和甲基紫 B 过程中及之后生成的中间化合物表明，这些染料的生物降解涉及到还原为隐色体、N-去甲基化以及三苯环的还原裂解。这些染料的 N-甲基化基团数量可能影响了它们的脱色率和三苯环的还原裂解。细胞毒性和抗菌测试数据表明，孔雀石绿和甲基紫 B 溶液（100mg/L）具有毒性。染料脱除颜色后毒性降低，但进一步孵育会导致毒性增加。
