Programa de Pós-Graduação em Ciências Morfológicas, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro-UFRJ, 21941-590, Rio de Janeiro, RJ, Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2010 Apr;151(3):343-50. doi: 10.1016/j.cbpc.2009.12.011. Epub 2010 Jan 7.
Crustaceans are interesting models to study the effects of ultraviolet (UV) radiation, and many species may be used as biomarkers for aquatic contamination of UV radiation reaching the surface of the Earth. Here, we investigated cell damage in the visual system of crabs Neohelice granulata that were acclimated to either 12L:12D, constant light, or constant dark, and were exposed to UVA or UVB at 12:00h (noon). The production of reactive oxygen species (ROS), antioxidant capacity against peroxyl radicals (ACAP), lipid peroxidation (LPO) damage, catalase activity, and pigment dispersion in the eye were evaluated. No significant differences from the three groups of controls (animals acclimated to 12L:12D, or in constant light, or not exposed to UV radiation) were observed in animals acclimated to 12L:12D, however, crabs acclimated to constant light and exposed to UV radiation for 30min showed a significant increase in ROS concentration, catalase activity, and LPO damage, but a decrease in ACAP compared with the controls. Crabs acclimated to constant darkness and exposed to UV for 30min showed a significantly increased ROS concentration and LPO damage, but the ACAP and catalase activity did not differ from the controls (animals kept in the dark while the experimental group was being exposed to UV radiation). Pigment dispersion in the pigment cells of eyes of animals acclimated to constant light was also observed. The results indicate that UVA and UVB alter specific oxidative parameters; however, the cell damage is more evident in animals deviated from the normal dark/light rhythm.
甲壳类动物是研究紫外线 (UV) 辐射影响的有趣模型，许多物种可被用作到达地球表面的 UV 辐射对水生生物污染的生物标志物。在这里，我们研究了适应 12L:12D、持续光照或持续黑暗的 Neohelice granulata 蟹视觉系统中的细胞损伤，并在 12:00h（中午）时暴露于 UVA 或 UVB。评估了活性氧 (ROS) 的产生、过氧自由基的抗氧化能力 (ACAP)、脂质过氧化 (LPO) 损伤、过氧化氢酶活性和眼睛中的色素分散。与适应 12L:12D、持续光照或未暴露于 UV 辐射的对照组相比，适应 12L:12D 的动物没有显示出显著差异，然而，适应持续光照并暴露于 UV 辐射 30min 的螃蟹显示 ROS 浓度、过氧化氢酶活性和 LPO 损伤显著增加，但与对照组相比，ACAP 降低。适应持续黑暗并暴露于 UV 辐射 30min 的螃蟹显示 ROS 浓度和 LPO 损伤显著增加，但 ACAP 和过氧化氢酶活性与对照组无差异（当实验组暴露于 UV 辐射时，动物处于黑暗中）。还观察到适应持续光照的动物眼睛色素细胞中的色素分散。结果表明，UVA 和 UVB 改变了特定的氧化参数；然而，在偏离正常黑暗/光照节律的动物中，细胞损伤更为明显。
