Baylor All Saints Medical Center, Baylor Research Institute, Fort Worth, TX 76104, USA.
Transplantation. 2010 Jan 15;89(1):47-54. doi: 10.1097/TP.0b013e3181be3bf2.
Although culturing islets before transplantation provides flexibility for evaluation of isolated islets and pretreatment of patients, it is well-known that isolated islets deteriorate rapidly in culture. In this study, we evaluated optimal temperature for culture/preservation of isolated human islets before transplantation.
Isolated islets were cultured or preserved for 48 hr in the following culture/preservation conditions: preservation at 4 degrees C in University of Wisconsin solution and culture at 22 degrees C or 37 degrees C in culture medium.
Islet morphology after 4 degrees C preservation was similar to that of fresh islets, whereas islet diameter after 37 degrees C or 22 degrees C culture was smaller than that of fresh islets. Islet yield significantly decreased at higher temperatures (24% loss in 37 degrees C culture and 19% loss in 22 degrees C culture, but <5% loss in 4 degrees C preservation). Cultured/preserved islets were transplanted into diabetic nude mice. The attainability of posttransplantation normoglycemia was significantly higher in the 4 degrees C preservation group than in 22 degrees C and 37 degrees C culture groups.
Preservation of isolated islets at 4 degrees C improves the outcome of islet transplantation more efficiently than preservation at 22 degrees C or 37 degrees C. Based on these data, we have performed short-time cold storage of isolated islets instead of culturing for current clinical islet transplantation.
尽管在移植前培养胰岛可提供评估分离胰岛和预处理患者的灵活性，但众所周知，分离胰岛在培养中会迅速恶化。在这项研究中，我们评估了移植前培养/保存分离人胰岛的最佳温度。
将分离的胰岛在以下培养/保存条件下培养或保存 48 小时：在威斯康星大学溶液中于 4°C 保存，或在 22°C 或 37°C 的培养基中培养。
4°C 保存后的胰岛形态与新鲜胰岛相似，而 37°C 或 22°C 培养后的胰岛直径小于新鲜胰岛。在较高温度下（37°C 培养时损失 24%，22°C 培养时损失 19%，但 4°C 保存时损失<5%）胰岛产量显著下降。培养/保存的胰岛被移植到糖尿病裸鼠体内。与 22°C 和 37°C 培养组相比，4°C 保存组胰岛移植后达到正常血糖的能力明显更高。
与 22°C 或 37°C 保存相比，4°C 保存分离胰岛更有效地改善胰岛移植的效果。基于这些数据，我们已经对分离胰岛进行了短时间的冷藏，而不是进行当前临床胰岛移植中的培养。
Zotero 插件