Fontana María Laura, Mroginski Luis Amado, Rey Hebe Yolanda
Instituto de Botánica del Nordeste (IBONE), Facultad de Ciencias Agrarias. Universidad Nacional del Nordeste, Corrientes, Argentina.
Biocell. 2009 Dec;33(3):179-86.
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 microM thidiazuron and 4.44 microM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 microM alpha-naphthalenacetic acid, 13.95 microM kinetin and 13.32 microM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 microM alpha-naphthalenacetic acid.
为了建立一种高效的植株再生方案，对绒毛落花生（Arachis villosa Benth.）的三个种质（S2866、S2867和L97）的小叶外植体在添加了不同植物生长调节剂组合的基本Murashige和Skoog培养基上进行培养，这些植物生长调节剂包括：α-萘乙酸、吲哚-3-丁酸、6-苄基腺嘌呤、激动素和噻苯隆。种质L97是唯一能够通过间接器官发生分化出芽的种质。芽再生的最适组合是添加了13.62微摩尔噻苯隆和4.44微摩尔6-苄基腺嘌呤的基本培养基。这些结果表明基因型在形态发生反应中的重要作用以及噻苯隆对绒毛落花生种质L97的器官发生效应。一种不含噻苯隆的培养基（仅添加了0.54微摩尔α-萘乙酸、13.95微摩尔激动素和13.32微摩尔6-苄基腺嘌呤）促进了再生芽的伸长。将离体芽转移到含有0.54微摩尔α-萘乙酸的生根培养基中90天后实现了不定根的形成。
