Darwish Wageh Sobhy, Ikenaka Yoshinori, Eldaly Elsaid Abozeid, Ohno Marumi, Sakamoto Kentaro Q, Fujita Shoichi, Ishizuka Mayumi
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.
J Vet Med Sci. 2010 May;72(5):561-6. doi: 10.1292/jvms.09-0318. Epub 2010 Jan 13.
The objective of this study was to investigate and characterize the metabolic activities of CYP1A in deer, cattle and horses in comparison to those of rats using ethoxyresorufin O-deethylation (EROD) and methoxyresorufin O-demethylation (MROD) assays. We performed an inhibition study for these activities using anti-rat CYP1A1 antibody and identified that these activities were due to the CYP1A subfamily. Interspecies differences in the CYP1A-dependent activities were highly observed in this study. In particular, we found that the horse had the highest EROD and MROD activities among the examined animal species. In the kinetic analysis, the horses showed the highest Vmax and catalytic efficiency (Vmax/Km), followed by the cattle, deer and rats.
本研究的目的是通过乙氧异吩唑酮 O-脱乙基酶（EROD）和甲氧基异吩唑酮 O-脱甲基酶（MROD）测定法，研究并表征鹿、牛和马中 CYP1A 的代谢活性，并与大鼠的代谢活性进行比较。我们使用抗大鼠 CYP1A1 抗体对这些活性进行了抑制研究，并确定这些活性归因于 CYP1A 亚家族。在本研究中，高度观察到了 CYP1A 依赖性活性的种间差异。特别是，我们发现马在所检查的动物物种中具有最高的 EROD 和 MROD 活性。在动力学分析中，马表现出最高的 Vmax 和催化效率（Vmax/Km），其次是牛、鹿和大鼠。
