Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, PR China.
Colloids Surf B Biointerfaces. 2010 Apr 1;76(2):549-55. doi: 10.1016/j.colsurfb.2009.12.017. Epub 2009 Dec 28.
Chitosan and heparin multilayer films were successfully constructed via layer-by-layer self assembly. These films were used as a polymeric template to synthesize silver nanoparticles. The silver concentration and nanoparticle size can be simply controlled by the assembly pH and loading pH, as demonstrated by UV-visible spectroscopy, transmission electron microscopy and atomic absorbance spectroscopy. The pH tunable uncompensated charge density within the multilayer films is believed to have great effect on the loading of silver ions, and then control the size and amount of silver nanoparticles within multilayer films. The antibacterial experiment shows that the silver nanoparticle-loaded chitosan/heparin multilayer films exhibit greatly enhanced antibacterial performance compared to the chitosan/heparin multilayer films without silver nanoparticles. In addition, the strong antibacterial property of silver nanoparticle-loaded films can last more than 1 month. Our method of in situ synthesis of metal nanoparticles in biocompatible multilayer films might provide great potential to design biofunctional nanocomposite films.
壳聚糖和肝素多层膜通过层层自组装成功构建。这些膜可用作合成银纳米粒子的聚合物模板。通过紫外可见光谱、透射电子显微镜和原子吸收光谱证明，银浓度和纳米颗粒尺寸可通过组装 pH 值和加载 pH 值简单控制。多层膜中 pH 值可调的未补偿电荷密度对银离子的加载有很大影响，从而控制多层膜中银纳米颗粒的尺寸和数量。抗菌实验表明，与不含银纳米粒子的壳聚糖/肝素多层膜相比，负载银纳米粒子的壳聚糖/肝素多层膜具有显著增强的抗菌性能。此外，负载银纳米粒子的薄膜具有超过 1 个月的强抗菌性能。我们在生物相容性多层膜中原位合成金属纳米粒子的方法可能为设计生物功能纳米复合薄膜提供巨大潜力。
