Zhu Xiaoli, Zhang Hongming, Lin Yong, Chen Pingsheng, Min Jie, Wang Zhengzhong, Xiao Wei, Chen Bao'an
Respiratory Diseases Department of Zhongda Hospital of Southeast University, Nanjing, Jiangsu Province, China.
J Chemother. 2009 Dec;21(6):666-72. doi: 10.1179/joc.2009.21.6.666.
Gambogic acid (GA) is one of the important active ingredients of gamboge. Our study examined the expression of transferrin receptors (TFR) on the cell surface of human lung SPC-A1 and SK-MES-1 cells and measured their GA-induced apoptosis rate. The results showed that SPC-A1 cells with a higher TFR expression were more sensitive at the same GA concentrations. To examine its distribution in cultured cells and study the mechanisms of apoptosis, we labeled GA with a (125)I tracer and examined the expression of apoptosis-related proteins. we found that GA uptake into SPC-A1 cells was higher than into SK-MES-1 cells; apoptosis-related proteins Caspase 2, Caspase 9, Caspase 10, Bax and p53 were involved in GA-induced apoptosis. We conclude that GA has an apoptosis-promoting effect on non small cell lung cancer cells. In clinical practice, the histopathological quantitation of TFR expression levels in tumor tissues may become a predictor of the sensitivity of patients' tumors to GA treatment.
藤黄酸（GA）是藤黄的重要活性成分之一。我们的研究检测了人肺SPC-A1和SK-MES-1细胞表面转铁蛋白受体（TFR）的表达，并测定了它们的GA诱导凋亡率。结果表明，在相同GA浓度下，TFR表达较高的SPC-A1细胞更敏感。为了检测其在培养细胞中的分布并研究凋亡机制，我们用（125）I示踪剂标记GA并检测凋亡相关蛋白的表达。我们发现SPC-A1细胞对GA的摄取高于SK-MES-1细胞；凋亡相关蛋白Caspase 2、Caspase 9、Caspase 10、Bax和p53参与了GA诱导的凋亡。我们得出结论，GA对非小细胞肺癌细胞有促凋亡作用。在临床实践中，肿瘤组织中TFR表达水平的组织病理学定量可能成为患者肿瘤对GA治疗敏感性的预测指标。
Zotero 插件