Wu Jian, Xue Xiaodong, Liu Junling, Si Xiaoqiang, Yang Guohu
Department of Burns and Plastic Surgery, People's Hospital of Gansu Province, Lanzhou Gansu, 730000, P.R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Dec;23(12):1482-6.
To investigate the effect of topical application of insulin on the burn wound healing in aging diabetes mellitus (DM) rats and to explore its mechanism.
Seventy-five SPF Wistar rats (female and/or male), aged 12-24 months and weighing 300-350 g, were selected and randomly divided into group A (burn control group, n=25), group B (DM burn control group, n=25), and group C (DM insulin treatment group, n=25). The rats in group B and group C were fed with high-fat, high-protein, and high-sugar forage for 1 month and received intraperitoneal injection of streptozotocin (STZ) to establish experimental model of aging DM. The rats were fed with high-fat, high-protein, and high-sugar forage for another 8 weeks. Then, the deep second-degree burn model was established in the rats of group B and group C. The wounds in group A and B underwent local subcutaneous injection of 2 mL isotonic saline and group C received local subcutaneous injection of 0.1 U insulin. The rate of wound healing was calculated 7, 14, and 21 days after burn injury. At 1, 3, 7, 14, and 21 days after burn injury, HE staining observation, immunohistochemistry staining for CD34, detection of sugar and hydroxyproline (HOP) content in wound tissue, and microvessel density (MVD) calculation were performed.
At 7, 14, and 21 days after burn injury, the wound healing rates of group A and group C was significantly higher than that of group B (P < 0.05), and there was no significant difference between group A and group C (P > 0.05). Histology observation at 21 days after burn injury: in group A, certain degree of epithelization was evident in the wound epithelium; in group B, large quantity of necrotic tissue was evident; in group C, complete epithelization occurred in the wound epithelium with better epithelial cell differentiation and more neonatal collagen. For the sugar content in the wound tissue, group A was significantly lower than group B or group C at 1, 3, 7, 14, and 21 days (P < 0.05) and group C was significantly lower than group B at 7, 14, and 21 days (P < 0.05). For the HOP content in the wound tissue and the MVD count, group A or group C was significantly higher than group B (P < 0.05) and there was no significant difference between group A and group C (P > 0.05). CD34 expression: in group A, it was (+) at 7 days, (++) at 14 days, and (+++) at 21 days; in group B, it was (+) at 14 and 21 days; in group C, it was (++) at 7 days and (+++) at 14 and 21 days.
Topical application of insulin can promote the synthesis of wound collagen, accelerate the wound angiogenesis, and speed up the wound healing in aging DM rats.
探讨局部应用胰岛素对老年糖尿病（DM）大鼠烧伤创面愈合的影响并探究其机制。
选取75只12 - 24月龄、体重300 - 350 g的SPF级Wistar大鼠（雌雄不限），随机分为A组（烧伤对照组，n = 25）、B组（DM烧伤对照组，n = 25）和C组（DM胰岛素治疗组，n = 25）。B组和C组大鼠给予高脂、高蛋白、高糖饲料喂养1个月，然后腹腔注射链脲佐菌素（STZ）以建立老年DM实验模型。之后继续给予高脂、高蛋白、高糖饲料喂养8周。然后，在B组和C组大鼠中建立深二度烧伤模型。A组和B组创面局部皮下注射2 mL等渗盐水，C组创面局部皮下注射0.1 U胰岛素。于烧伤后7、14和21天计算创面愈合率。在烧伤后1、3、7、14和21天，进行HE染色观察、CD34免疫组化染色、检测创面组织中糖和羟脯氨酸（HOP）含量以及计算微血管密度（MVD）。
烧伤后7、14和21天，A组和C组的创面愈合率显著高于B组（P < 0.05），A组和C组之间无显著差异（P > 0.05）。烧伤后21天组织学观察：A组创面上皮有一定程度的上皮化；B组可见大量坏死组织；C组创面上皮完全上皮化，上皮细胞分化较好，新生胶原较多。对于创面组织中的糖含量，A组在1、3、7、14和21天均显著低于B组或C组（P < 0.05），C组在7、14和21天显著低于B组（P < 0.05）。对于创面组织中的HOP含量和MVD计数，A组或C组显著高于B组（P < 0.05），A组和C组之间无显著差异（P > 0.05）。CD34表达：A组7天为（+），14天为（++），21天为（+++）；B组14天和21天为（+）；C组7天为（++），14天和21天为（+++）。
局部应用胰岛素可促进老年DM大鼠创面胶原蛋白合成，加速创面血管生成，加快创面愈合。
Zotero 插件