Center for Women's Health Research, Department of Obstetrics and Gynecology, Meharry Medical College, Nashville, TN 37208, USA.
Am J Obstet Gynecol. 2010 Mar;202(3):289.e1-9. doi: 10.1016/j.ajog.2009.10.885. Epub 2010 Jan 13.
The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model.
ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment.
Inhibitory effect of EGCG (200 micromol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At > or = 50 micromol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05).
EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.
本研究旨在探讨表没食子儿茶素没食子酸酯(EGCG)对体外培养的大鼠平滑肌瘤(ELT3)细胞及裸鼠模型的作用。
用不同浓度的 EGCG 处理 ELT3 细胞。评估细胞增殖、增殖细胞核抗原(PCNA)和周期蛋白依赖性激酶 4(Cdk4)蛋白水平。将 ELT3 细胞皮下接种于雌性无胸腺裸鼠。动物每天饮用 1.25 毫克 EGCG(在饮用水中)。治疗 4 和 8 周后收集和评估肿瘤。
EGCG(200 μmol/L)处理 24 小时后对 ELT3 细胞表现出抑制作用(P<0.05)。≥50 μmol/L 时,EGCG 显著降低 PCNA 和 Cdk4 蛋白水平(P<0.05)。体内,EGCG 治疗在治疗后 4 和 8 周时显著降低肿瘤的体积和重量(P<0.05)。EGCG 治疗组的 PCNA 和 Cdk4 蛋白水平明显降低(P<0.05)。
EGCG 有效抑制大鼠 ELT3 子宫平滑肌瘤细胞的体外和体内增殖,并诱导其凋亡。