绿茶提取物抑制体外培养的子宫肌瘤细胞及裸鼠子宫肌瘤的生长。
Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice.
机构信息
Center for Women's Health Research, Department of Obstetrics and Gynecology, Meharry Medical College, Nashville, TN 37208, USA.
出版信息
Am J Obstet Gynecol. 2010 Mar;202(3):289.e1-9. doi: 10.1016/j.ajog.2009.10.885. Epub 2010 Jan 13.
Abstract
OBJECTIVE
The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model.
STUDY DESIGN
ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment.
RESULTS
Inhibitory effect of EGCG (200 micromol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At > or = 50 micromol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05).
CONCLUSION
EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.
摘要
目的
本研究旨在探讨表没食子儿茶素没食子酸酯（EGCG）对体外培养的大鼠平滑肌瘤（ELT3）细胞及裸鼠模型的作用。
研究设计
用不同浓度的 EGCG 处理 ELT3 细胞。评估细胞增殖、增殖细胞核抗原（PCNA）和周期蛋白依赖性激酶 4（Cdk4）蛋白水平。将 ELT3 细胞皮下接种于雌性无胸腺裸鼠。动物每天饮用 1.25 毫克 EGCG（在饮用水中）。治疗 4 和 8 周后收集和评估肿瘤。
结果
EGCG（200 μmol/L）处理 24 小时后对 ELT3 细胞表现出抑制作用（P<0.05）。≥50 μmol/L 时，EGCG 显著降低 PCNA 和 Cdk4 蛋白水平（P<0.05）。体内，EGCG 治疗在治疗后 4 和 8 周时显著降低肿瘤的体积和重量（P<0.05）。EGCG 治疗组的 PCNA 和 Cdk4 蛋白水平明显降低（P<0.05）。
结论
EGCG 有效抑制大鼠 ELT3 子宫平滑肌瘤细胞的体外和体内增殖，并诱导其凋亡。
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