Liu Y P, Wong V G, Chabner B A
J Natl Cancer Inst. 1977 Dec;59(6):1605-9. doi: 10.1093/jnci/59.6.1605.
3':5'-Cyclic-AMP phosphodiesterase (EC 3.1.4.17) and the activating factor of cyclic nucleotide phosphodiesterase were detected in cultured human cell lines from patients with lymphoblastic leukemia and retinoblastoma and in the Brown-Pearce (rabbit) carcinoma. The homogenate of lymphoblasts contained levels of the activating factor in excess of that required to produce maximal activation of the endogenous phosphodiesterase. The activating factor found in these malignant cells appears to be similar to the calcium-binding protein activator of bovine brain phosphodiesterase on the basis of the molecular weight obtained from gel filtration, electrophoretic patterns, calcium requirement for the activity, and the effect of calcium on the proteolysis. In addition, the tumor-derived activator was able to restore the activity of activator-deficient phosphodiesterase from the bovine brain.
3':5'-环磷酸腺苷磷酸二酯酶（EC 3.1.4.17）和环核苷酸磷酸二酯酶激活因子在来自淋巴细胞白血病和视网膜母细胞瘤患者的培养人细胞系以及布朗-皮尔斯（兔）癌中被检测到。成淋巴细胞匀浆中激活因子的水平超过了使内源性磷酸二酯酶产生最大激活所需的水平。基于从凝胶过滤获得的分子量、电泳图谱、活性对钙的需求以及钙对蛋白水解的影响，在这些恶性细胞中发现的激活因子似乎与牛脑磷酸二酯酶的钙结合蛋白激活剂相似。此外，肿瘤来源的激活剂能够恢复牛脑来源的激活剂缺陷型磷酸二酯酶的活性。
