Yue Bingfang, Kushnir Mark M, Urry Francis M, Rockwood Alan L
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.
Methods Mol Biol. 2010;603:389-98. doi: 10.1007/978-1-60761-459-3_38.
We describe a method for the quantitative analysis of nicotine, cotinine, trans-3'-hydroxy cotinine, nornicotine, and anabasine in urine, serum, and plasma using liquid chromatography-tandem mass spectrometry. A mix of deuterium-labeled internal standards (IS) is added to a specimen aliquot. The aliquot is extracted using mixed-mode solid phase extraction and eluted into an autosampler vial for injection into an LC-MS-MS system. An Atlantis silica column is used for LC separation in hydrophilic interaction mode. Tandem mass spectrometry detection is performed in positive ion mode with electrospray ionization and two multiple reaction monitoring (MRM) transitions monitored for each analyte and IS.
我们描述了一种使用液相色谱 - 串联质谱法对尿液、血清和血浆中的尼古丁、可替宁、反式 - 3'-羟基可替宁、去甲烟碱和新烟草碱进行定量分析的方法。将氘代内标（IS）混合物添加到一份标本等分试样中。该等分试样使用混合模式固相萃取进行提取，并洗脱到自动进样器小瓶中，以便注入液相色谱 - 串联质谱系统。使用Atlantis硅胶柱以亲水相互作用模式进行液相色谱分离。串联质谱检测在正离子模式下进行，采用电喷雾电离，对每种分析物和内标监测两个多反应监测（MRM）转换。
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