Zhang Yong, Liu Jian-Feng, Wang Dong-Yan
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhongguo Yi Miao He Mian Yi. 2009 Feb;15(1):35-40.
To study the molecular evolution of Human enterovirus 71 (HEV71) isolated from clinical specimens of Hand, Foot and Mouth Disease (HFMD)patients in Gansu in 2008.
Clinical samples including stools, throat swabs and vesicle fluids were collected from HFMD patients in outpatient service in Gansu province and then viral isolation was performed. Reverse Transcription-Polymerase Chain Reactions (RT-PCR) specific for HEV71 were performed with the viral isolates. Then 20 HEV71 isolates were selected randomly for entire VP1 coding gene amplification and sequencing, finally phylogenetic tree was constructed among these 20 Gansu HEV71 strains and 38 HEV71 representative strains of known genotypes and subgenotypes.
The nucleotide acid and amino acid of 20 Gansu HEV71 strains were closed to HEV71 strains isolated in Mainland China since 1998, especially has the highest homology to HEV71 stains circulated in mainland China in 2008 and it showed that all strains clustered within the C4b evolution branch of C4 Subgenotype. But there was some difference in the nucleotide and the amino acid in VP1 region among the 20 Gansu HEV71 strains, the homology were 92.5%-100.0% and 96.9%-100% respectively. 20 HEV71 strains lie in several lineages in the phylogenetic tree, and it revealed that these strains belonged to at least 7 viral transmission chains.
The HEV71 strains circulated in Gansu province in 2008 were all belong to C4b evolution branch of C4 subgenotype. Phylogenetic analysis revealed these HEV71 strains located in different lineages,and a close phylogenetic and chronological relationship with HEV71 isolated in other provinces in mainland China had been observed in 2008. This confirmed Gansu HEV71 strains had not evolved independently, but coevolved with the HEV71 strains in other provinces in mainland China.
研究2008年从甘肃省手足口病（HFMD）患者临床标本中分离出的人肠道病毒71型（HEV71）的分子进化情况。
从甘肃省门诊的手足口病患者中采集粪便、咽拭子和水疱液等临床样本，然后进行病毒分离。对病毒分离株进行针对HEV71的逆转录-聚合酶链反应（RT-PCR）。随后随机选择20株HEV71分离株进行完整VP1编码基因的扩增和测序，最后在这20株甘肃HEV71毒株与38株已知基因型和亚基因型的HEV71代表性毒株之间构建系统发育树。
20株甘肃HEV71毒株的核苷酸和氨基酸与1998年以来在中国内地分离出的HEV71毒株相近，尤其与2008年在中国内地流行的HEV71毒株同源性最高，且显示所有毒株都聚集在C4亚基因型的C4b进化分支内。但20株甘肃HEV71毒株在VP1区域的核苷酸和氨基酸存在一些差异，同源性分别为92.5%-100.0%和96.9%-100%。20株HEV71毒株在系统发育树中位于几个分支，表明这些毒株至少属于7个病毒传播链。
2008年在甘肃省流行的HEV71毒株均属于C4亚基因型的C4b进化分支。系统发育分析显示这些HEV71毒株位于不同分支，且在2008年观察到与中国内地其他省份分离出的HEV71有密切的系统发育和时间关系。这证实甘肃HEV71毒株并非独立进化，而是与中国内地其他省份的HEV71毒株共同进化。
