Department of Chemistry, Universite de Montreal, Montreal, Quebec, Canada.
J Agric Food Chem. 2010 Feb 10;58(3):1442-6. doi: 10.1021/jf903362v.
Sulfonamides are antibiotic compounds widely used in the dairy industry. Their presence in diary milk poses a risk to public health and may also contribute to the spread of antibiotic resistance in bacteria. Sulfonamide residues in dairy milk were quantified by tandem mass spectrometry (MS/MS) using a novel ionization source based on laser diode thermal desorption-negative mode atmospheric pressure chemical ionization (LDTD-APCI(-)). Seven sulfonamides spiked in milk were extracted with acetonitrile, which yielded high recoveries (77.5-101.5%). Calibration curves in the matrix showed good linearity (0.9977 >or= R(2) >or= 0.9658) over the dynamic range (1.6-500 microg L(-1)), and limits of quantitation were between 2 and 14 microg L(-1), lower than or of the same magnitude as maximum residue criteria set by several regulatory agencies (10-100 ng L(-1)). In addition, the run time using the LDTD-MS/MS system was 30 s per sample, as compared to actual methods running from 7 to 84 min for the same sulfonamide residue compounds, which gave the method the high screening throughput capacity necessary for monitoring milk production.
磺胺类药物是在乳品行业中广泛使用的抗生素化合物。它们在牛奶中的存在对公众健康构成了威胁，也可能导致细菌对抗生素耐药性的传播。本研究采用基于激光二极管热解吸-负大气压化学电离（LDTD-APCI(-)）的新型离子源，通过串联质谱（MS/MS）对牛奶中的磺胺类药物残留进行了定量分析。向牛奶中添加 7 种磺胺类药物，用乙腈提取，回收率高（77.5%-101.5%）。在基质中，校准曲线的动态范围（1.6-500μg/L）具有良好的线性（0.9977≥R(2)≥0.9658），定量限在 2-14μg/L 之间，低于或与几个监管机构设定的最大残留限量标准（10-100ng/L）相同。此外，与实际方法相比，使用 LDTD-MS/MS 系统的运行时间为每个样品 30s，而相同磺胺类药物残留化合物的实际方法运行时间为 7-84min，这使得该方法具有监测牛奶生产所需的高通量筛选能力。
