Nakahara Y, Takahashi K, Shimamine M, Takeda Y
Narcotics Section, National Institute of Hygiene Sciences, Tokyo, Japan.
J Forensic Sci. 1991 Jan;36(1):70-8.
Determination of methamphetamine and amphetamine in hair was performed by gas chromatography/mass spectrometry using stable isotope-labeled internal standards, 2-methylamino-1-phenylpropane-2,3,3,3-d4 and 2-amino-1-phenylpropane-2,3,3,3-d4. Extraction of hair with methanol/5M hydrochloric acid (20:1) using ultrasonication was chosen as the standard method. The calibration curves for amphetamines in the hair were linear from 1 to 100 ng/mg (r greater than 0.99). The detection limit was 0.5 ng/mg at the 95% confidence level. The coefficients of variation (CV) (n = 8) of analysis using the spiked hair with methamphetamine were from 0.7 to 6%. The CV (n = 8) of analysis of the methamphetamine abuser's hair was 17.5%. Sectional analysis of monkey and human hair after methamphetamine ingestion suggested a good correlation between the duration of drug use and drug distribution in the hair.
采用气相色谱/质谱联用技术，以稳定同位素标记的内标物2-甲基氨基-1-苯基丙烷-2,3,3,3-d4和2-氨基-1-苯基丙烷-2,3,3,3-d4测定毛发中的甲基苯丙胺和苯丙胺。选择用甲醇/5M盐酸（20:1）超声提取毛发作为标准方法。毛发中苯丙胺类药物的校准曲线在1至100 ng/mg范围内呈线性（r大于0.99）。在95%置信水平下，检测限为0.5 ng/mg。用甲基苯丙胺加标毛发进行分析的变异系数（CV）（n = 8）为0.7%至6%。甲基苯丙胺滥用者毛发分析的CV（n = 8）为17.5%。对摄入甲基苯丙胺后的猴毛和人发进行分段分析表明，药物使用时长与毛发中药物分布之间具有良好的相关性。
