Center for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, No. 253 Gong ye da dao zhong, Guangzhou 510282, People's Republic of China.
Virology. 2010 Mar 15;398(2):290-8. doi: 10.1016/j.virol.2009.12.010. Epub 2010 Jan 15.
The dengue virus (DENV) nonstructural protein 1 (NS1) is an immunogenic protein that holds potential for the development of vaccines and diagnostic reagents; however, the epitopes of NS1 have not been comprehensively mapped. We mapped B-cell linear epitopes on NS1 using 149 monoclonal antibodies with DENV serotype specificity and cross-reactivity as well as antisera from 27 mice immunized with the four DENV serotypes. Epitope recognition analysis was performed using a set of 15-mer sequential overlapping peptides that spanned the entire NS1 protein from DENV-1. This strategy identified three regions of NS1 that are DENV-1 serotype-specific epitopes, namely amino acid residues 1-15, 71-85, and 338-352. We also identified five group-specific B-cell epitopes that were highly conserved among isolates of the four DENV serotypes. These novel immunodominant serotype- and group-specific B-cell epitopes of DENV NS1 may aid the development of new dengue vaccines and diagnostic assays.
登革热病毒（DENV）非结构蛋白 1（NS1）是一种免疫原性蛋白，具有开发疫苗和诊断试剂的潜力；然而，NS1 的表位尚未得到全面描绘。我们使用具有 DENV 血清型特异性和交叉反应性的 149 种单克隆抗体以及来自 27 只用四种 DENV 血清型免疫的小鼠的抗血清，对 NS1 上的 B 细胞线性表位进行了作图。使用一组跨越 DENV-1 整个 NS1 蛋白的 15 个氨基酸顺序重叠肽进行了表位识别分析。该策略确定了三个 DENV-1 血清型特异性表位的 NS1 区域，即氨基酸残基 1-15、71-85 和 338-352。我们还鉴定了五个在四个 DENV 血清型分离株中高度保守的群体特异性 B 细胞表位。这些新型免疫显性血清型和群体特异性 DENV NS1 B 细胞表位可能有助于新的登革热疫苗和诊断检测方法的开发。
