School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan.
Virus Res. 2010 Apr;149(1):109-14. doi: 10.1016/j.virusres.2009.12.007. Epub 2010 Jan 15.
Capsium spp. are an important vegetable crop cultivated through Pakistan. Leaf curl disease is the major disease of Capsicum spp. in Pakistan caused by viruses. The disease has previously been shown to be associated with begomoviruses and betasatellites. We have cloned and sequenced a begomovirus and its associated betasatellite from Capsicum originating from central Pakistan. The begomovirus isolated was distinct from all previously characterised viruses and we propose the name Pepper leaf curl Lahore virus (PepLCLV) for this new species. Comparison of the sequence of PepLCLV with previously characterised begomoviruses shows it likely to have resulted from recombination between Papaya leaf curl virus and Chili leaf curl virus (ChiLCV), two species that have previously been identified in Pakistan. The betasatellite associated with PepLCLV in Capsicum was identified as Chili leaf curl betasatellite (ChLCB). This is the first identification of a cognate begomovirus for ChLCB infecting Capsicum, although this betasatellite has been shown in association with ChiLCV infecting potato in Pakistan. PepLCLV is one of an increasing number of monopartite begomoviruses shown to be associated with a betasatellite and one of the numerous species that affect Capsicum. In view of their only having been identified in Pakistan, PepLCLV and ChLCB likely represent a geographically distinct, Capsicum adapted, begomovirus-betasatellite complex.
辣椒是巴基斯坦重要的蔬菜作物。辣椒曲叶病是巴基斯坦辣椒的主要病害，由病毒引起。以前的研究表明，这种病与双生病毒和卫星β病毒有关。我们从巴基斯坦中部采集的辣椒中克隆并测序了一种双生病毒及其相关的卫星β病毒。分离出的双生病毒与以前鉴定的所有病毒都不同，我们提议将这种新病毒命名为辣椒曲叶卷叶病毒（PepLCLV）。PepLCLV 与以前鉴定的双生病毒序列的比较表明，它可能是由木瓜曲叶病毒和辣椒曲叶病毒（ChiLCV）重组产生的，这两种病毒以前在巴基斯坦被发现过。与 PepLCLV 在辣椒中相关的卫星β病毒被鉴定为辣椒曲叶卷曲卫星β（ChLCB）。这是首次鉴定出与 ChLCB 相关的辣椒曲叶卷曲卫星β感染辣椒的同源双生病毒，尽管这种卫星β病毒已经被证明与感染巴基斯坦马铃薯的 ChiLCV 有关。PepLCLV 是越来越多的单分体双生病毒之一，这些病毒与卫星β病毒有关，是影响辣椒的众多病毒之一。鉴于它们只在巴基斯坦被发现，PepLCLV 和 ChLCB 可能代表了一种在地理上独特的、适应于辣椒的双生病毒-卫星β病毒复合体。
