Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 88100 Catanzaro, Italy.
Talanta. 2010 Feb 15;80(4):1532-8. doi: 10.1016/j.talanta.2009.03.060. Epub 2009 Apr 5.
Promising profiling techniques based on new material/solid phase extraction for capturing "molecular signatures" from body fluids are being coupled to MALDI-TOF-MS. Sample preparation significantly influences spectrum quality in this ionization method. Mesoporous silica beads (MSB), by the means of nano-sized porous channels with high surface area, enable harvesting of peptides from plasma and serum excluding large size proteins. We have investigated the morphology of a sample slurry, developed as a new tool for plasma peptides enrichment based on mesoporous materials. Our study highlights a correlation between crystals morphology and enhanced performances in MALDI-TOF-MS analysis. This is the first report which correlates the increase in signal intensity with crystal formation in samples preparations which make use of various kinds of slurries for the analysis of samples clinically relevant like human plasma.
基于新材料/固相萃取的有前途的分析技术，用于从体液中捕获“分子特征”，正与 MALDI-TOF-MS 相结合。在这种离子化方法中，样品制备会显著影响光谱质量。中孔硅珠（MSB）通过具有高表面积的纳米级多孔通道，可以从血浆和血清中提取肽，同时排除大尺寸蛋白质。我们研究了作为基于中孔材料的新型血浆肽富集工具的样品悬浮液的形态。我们的研究强调了晶体形态与 MALDI-TOF-MS 分析中性能增强之间的相关性。这是第一个将信号强度的增加与样品制备中晶体形成相关联的报告，该报告使用了各种悬浮液来分析临床上相关的样品，如人血浆。
