Nikièma A, Toé L, Adjami G, Ouédraogo Traoré R
Université de Ouagadougou, 3, BP 7021 Ouagadougou 03,Burkina Faso.
Bull Soc Pathol Exot. 2010 Feb;103(1):8-13. doi: 10.1007/s13149-009-0003-x.
A prospective study (from August 2006 to April 2007) was carried out with 214 cerebrospinal fluid samples with suspicion of bacterial meningitis. The aim of the study was to assess the effectiveness of the simultaneous detection of Neisseria meningitidis, Streptococcus sp. and Haemophilus influenzae using seminested polymerase chain reaction strategy. Among the 214 samples tested by both PCR and culture, the overall confirmation rate was 64% for PCR and 40.1% for culture (P = 2 x 10⁻⁶). Taking culture method as the standard reference, the overall sensitivity of PCR was 98.8% and specificity, 59.4%. The sensitivity of PCR was 100, 97.3 and 100% respectively for N. meningitidis, Streptococcus sp. and H. influenzae with respective specificities of 70, 93.2 and 97.2%. In conclusion, the seminested PCR strategy is a sensitive method and it can be implemented in the reference public health laboratories for an exhaustive microbiological surveillance of bacterial meningitis.
一项前瞻性研究（2006年8月至2007年4月）纳入了214份疑似细菌性脑膜炎的脑脊液样本。该研究旨在评估采用半巢式聚合酶链反应策略同时检测脑膜炎奈瑟菌、链球菌属和流感嗜血杆菌的有效性。在214份同时采用PCR和培养法检测的样本中，PCR的总体确诊率为64%，培养法为40.1%（P = 2×10⁻⁶）。以培养法作为标准参考，PCR的总体敏感性为98.8%，特异性为59.4%。对于脑膜炎奈瑟菌、链球菌属和流感嗜血杆菌，PCR的敏感性分别为100%、97.3%和100%，特异性分别为70%、93.2%和97.2%。总之，半巢式PCR策略是一种敏感的方法，可在参考公共卫生实验室实施，用于对细菌性脑膜炎进行全面的微生物学监测。
