Laser Dynamics Laboratory, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.
J Am Chem Soc. 2010 Feb 10;132(5):1517-9. doi: 10.1021/ja9102698.
By properly conjugating gold nanoparticles with specific peptides, we were successful in selectively transporting them to the nuclei of cancer cells. Confocal microscopy images of DNA double-strand breaks showed that localization of gold nanoparticles at the nucleus of a cancer cell damages the DNA. Gold nanoparticle dark-field imaging of live cells in real time revealed that the nuclear targeting of gold nanoparticles specifically induces cytokinesis arrest in cancer cells, where binucleate cell formation occurs after mitosis takes place. Flow cytometry results indicated that the failure to complete cell division led to programmed cell death (apoptosis) in cancer cells. These results show that gold nanoparticles localized at the nuclei of cancer cells have important implications in understanding the interaction between nanomaterials and living systems.
通过将金纳米粒子与特定的肽适当缀合，我们成功地将其选择性地输送到癌细胞的细胞核中。DNA 双链断裂的共焦显微镜图像表明，金纳米粒子在癌细胞核内的定位会破坏 DNA。实时活细胞的金纳米粒子暗场成像显示，金纳米粒子的核靶向特异性诱导癌细胞的胞质分裂停滞，有丝分裂后会形成双核细胞。流式细胞术结果表明，细胞分裂失败导致癌细胞发生程序性细胞死亡（细胞凋亡）。这些结果表明，定位于癌细胞核内的金纳米粒子在理解纳米材料与生命系统的相互作用方面具有重要意义。
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