iPS 细胞生成的基因传递系统。
Gene-delivery systems for iPS cell generation.
机构信息
Maine Medical Center Research Institute, Maine Medical Center, COBRE in Stem Biology and Regenerative Medicine, 81 Research Drive, Scarborough, Maine 04074, USA.
出版信息
Expert Opin Biol Ther. 2010 Feb;10(2):231-42. doi: 10.1517/14712590903455989.
IMPORTANCE OF THE FIELD
Induced pluripotent stem (iPS) cells offer extraordinary promise for regenerative medicine applications, and provide new opportunities for use in disease modeling, drug screening and drug toxicology. AREAS COVED IN THIS REVIEW: iPS cell technology is still in its infancy. In this review article, we present a comprehensive survey of reprogramming approaches focusing on gene-delivery systems used for generation of iPS cells from somatic cells, categorize gene-delivery vectors, and discuss their advantages and limitations for somatic cell reprogramming. We include pertinent literature published between 2006 and the present.
WHAT THE READER WILL GAIN
Although iPS cell technology has been improved via the use of various gene-delivery vectors, it still suffers from either low reprogramming efficiency or too many genomic modification steps. Extensive work is still required to improve current vectors or explore new vectors for effectively reprogramming human somatic cells into iPS cells, with or without minimal genomic modification steps.
TAKE HOME MESSAGE
A single non-integrating reprogramming vector system with high reprogramming efficiency is probably essential for generation of clinically translatable human iPS cells.
重要性的领域
诱导多能干细胞（iPS）细胞为再生医学应用提供了非凡的前景，并为疾病建模、药物筛选和药物毒理学的应用提供了新的机会。
本篇综述涵盖的领域
iPS 细胞技术仍处于起步阶段。在这篇综述文章中，我们全面调查了重编程方法，重点介绍了用于从体细胞生成 iPS 细胞的基因传递系统，对基因传递载体进行分类，并讨论了它们在体细胞重编程中的优缺点。我们包括了 2006 年至今发表的相关文献。
读者将获得的收益
尽管 iPS 细胞技术已经通过使用各种基因传递载体得到了改进，但它仍然存在重编程效率低或基因组修饰步骤过多的问题。仍然需要做大量的工作来改进现有的载体或探索新的载体，以便有效地将人类体细胞重编程为 iPS 细胞，无论是否有最小的基因组修饰步骤。
需要吸取的教训
具有高效重编程效率的单一非整合重编程载体系统可能是生成临床上可转化的人类 iPS 细胞所必需的。
Zotero 插件