细胞转化与分化。劳氏肉瘤病毒转化对鸡软骨细胞硫酸化蛋白聚糖合成的影响。
Cellular transformation and differentiation. Effect of Rous sarcoma virus transformation on sulfated proteoglycan synthesis by chicken chondrocytes.
作者信息
Muto M, Yoshimura M, Okayama M, Kaji A
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4173-7. doi: 10.1073/pnas.74.10.4173.
Incorporation of sulfate into sulfated proteoglycans by isolated chicken chondrocytes was inhibited up to 74% by transformation with the Rous sarcoma virus, and a similar inhibitory effect was observed on acetate incorporation into chondroitin sulfate. Slower sedimenting sulfated proteoglycans appear after the viral transformation. The ratio of chondroitin 4-sulfate to chondroitin 6-sulfate in these slower sedimenting sulfated proteoglycans was different from that of normal chondrocytes, but the chain lengths of sulfated glycosaminoglycans produced by normal chondrocytes and transformed chondrocytes were not significantly different. Chondrocytes were also infected with a temperature-sensitive mutant of RSV, ts LA24, which has a temperature-sensitive lesion in the transforming gene. Hyaluronic acid production by these cells was increased, and the slower sedimenting sulfated proteoglycan was produced only at the permissive temperature.
用劳斯肉瘤病毒转化后，离体鸡软骨细胞将硫酸盐掺入硫酸化蛋白聚糖的过程受到高达74%的抑制，并且在乙酸盐掺入硫酸软骨素的过程中也观察到了类似的抑制作用。病毒转化后出现沉降较慢的硫酸化蛋白聚糖。这些沉降较慢的硫酸化蛋白聚糖中硫酸软骨素4-硫酸盐与硫酸软骨素6-硫酸盐的比例与正常软骨细胞不同，但正常软骨细胞和转化软骨细胞产生的硫酸化糖胺聚糖的链长没有显著差异。软骨细胞还用劳斯肉瘤病毒的温度敏感突变体ts LA24进行感染，该突变体在转化基因中有温度敏感损伤。这些细胞产生的透明质酸增加，并且只有在允许温度下才产生沉降较慢的硫酸化蛋白聚糖。
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