Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Handian District, 100093, Beijing, China.
Amino Acids. 2010 Jun;39(1):181-94. doi: 10.1007/s00726-009-0397-6. Epub 2009 Nov 29.
Proteome patterns in peach fruit (Prunus persica L.) stored at different low temperatures were examined in order to gain a better understanding why peach fruit is less prone to chilling injury when stored at 0 degrees C than at 5 degrees C. Some differently expressed proteins in peach fruit stored at 0 and 5 degrees C were identified using electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Among these proteins, four membrane stability related proteins, i.e., enolase, temperature-induced lipocalin, major allergen Pru p 1, and type II SK2 dehydrin were enhanced, but three proteins related to phenolic compounds metabolization, cinnamyl-alcohol dehydrogenase 5, cinnamyl-alcohol dehydrogenase 1, and chorismate mutase, were repressed in peach fruit at 0 degrees C as compared to that at 5 degrees C. The abundance of glucose-6-phosphate dehydrogenase, NADP-dependent isocitrate dehydrogenase, and NADP-dependent malic enzyme, which catalyze the reactions during sugar metabolism and energy pathways, was found to decrease in peach fruit stored at 0 degrees C. In addition, our data revealed that low temperature of 0 degrees C might regulate the endogenous H(2)O(2) level, resulting in activating the transcriptional level of genes encoding the proteins related to membrane stability. These results provide a comprehensive knowledge to understand the mechanisms by which peach fruit stored at 0 degrees C showed a higher chilling tolerance than that at 5 degrees C.
为了更好地理解为什么桃果实在 0°C 下贮藏比在 5°C 下贮藏时不易发生冷害，研究了不同低温下桃果实的蛋白质组模式。采用电喷雾电离四极杆飞行时间串联质谱法鉴定了在 0°C 和 5°C 下贮藏的桃果实中一些表达不同的蛋白质。在这些蛋白质中，四种与膜稳定性相关的蛋白质，即烯醇酶、温度诱导的脂蛋白、主要过敏原 Pru p 1 和 II 型 SK2 脱水素增强，而三种与酚类化合物代谢相关的蛋白质，肉桂醇脱氢酶 5、肉桂醇脱氢酶 1 和色氨酸合酶，在 0°C 下贮藏的桃果实中受到抑制，而在 5°C 下贮藏的桃果实中受到抑制。在 0°C 下贮藏的桃果实中，催化糖代谢和能量途径中反应的葡萄糖-6-磷酸脱氢酶、NADP 依赖性异柠檬酸脱氢酶和 NADP 依赖性苹果酸酶的丰度降低。此外，我们的数据表明，0°C 的低温可能调节内源性 H₂O₂水平，从而激活与膜稳定性相关的蛋白质编码基因的转录水平。这些结果为理解 0°C 贮藏的桃果实比 5°C 贮藏时具有更高的冷耐性的机制提供了全面的知识。
