IEF 和 LC-MALDI-TOF/TOF 相结合的 shotgun 质谱工作流程。
Shotgun mass spectrometry workflow combining IEF and LC-MALDI-TOF/TOF.
机构信息
Max Planck Institute of Psychiatry, Proteomics and Biomarkers, Kraepelinstrasse 2, 80804, Munich, Germany.
出版信息
Protein J. 2010 Feb;29(2):99-102. doi: 10.1007/s10930-009-9227-7.
Abstract
We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the 'Isotope-Coded Protein Labeling' (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches.
摘要
我们提出了一种高通量的 shotgun 质谱工作流程，该流程使用二维肽分级程序，包括等电聚焦和 RP-HPLC，然后进行质谱分析，旨在优化肽分离和蛋白质鉴定。作为工作流程的一部分，我们使用了“同位素编码蛋白质标记”（ICPL）方法来准确相对定量蛋白质表达。该工作流程已成功应用于精神分裂症与健康对照脑组织的比较蛋白质组学分析，可以作为蛋白质组学研究的一种替代方法。
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