Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
J Biomater Sci Polym Ed. 2010;21(2):225-36. doi: 10.1163/156856209X415549.
The objective of this study is to prepare a non-viral carrier of gene expression from the polysaccharide dextran and evaluate the effect of amine compounds introduced to dextran on the level of gene expression. Dextran with a molecular weight of 74 x 10(3) was cationized by the chemical introduction of different amine compounds. The cationized dextran was complexed with a plasmid DNA and the vitro gene transfection was investigated for HeLa cells. The level of gene expression depended on the amine compound introduced to dextran. The highest level was observed for the complex of spermine-introduced dextran and plasmid DNA. The highest cellular internalization and the best buffering effect were observed among every cationized dextran. Every complex did not show any cytotoxicity. It is concluded that the superior properties of spermine-introduced dextran enabled the plasmid DNA to enhance the expression level to a great extent compared with other cationized dextrans. Cationized dextran is a promising non-viral carrier of plasmid DNA.
本研究的目的是从多糖葡聚糖制备非病毒基因表达载体，并评估引入葡聚糖的胺化合物对基因表达水平的影响。通过化学引入不同的胺化合物，将分子量为 74 x 10(3)的葡聚糖阳离子化。将阳离子化的葡聚糖与质粒 DNA 复合，并对 HeLa 细胞进行体外基因转染。基因表达水平取决于引入葡聚糖的胺化合物。在带正电荷的葡聚糖和质粒 DNA 的复合物中观察到最高水平。在每一种带正电荷的葡聚糖中，都观察到了最高的细胞内化和最佳的缓冲效果。每一种复合物都没有显示出任何细胞毒性。结论是，引入的 spermine 赋予了带正电荷的葡聚糖优异的性质，使质粒 DNA 的表达水平相对于其他带正电荷的葡聚糖有了很大的提高。阳离子化葡聚糖是一种很有前途的质粒 DNA 的非病毒载体。
